天津中医药Issue(2)：111-113,3.DOI:10.11656/j.issn.1672-1519.2016.02.13
栀子苷预处理对 H9C2心肌细胞缺氧/复氧损伤的影响及机制研究
Effects and mechanism of geniposide pretreatment on H9C2 cardiomyocytes during hypoxia/reoxygenation injury
摘要
Abstract
Objective] To explore the effects and mechanism of geniposide pretreatment on H9C2 cardiomyocytes during hypoxia/reoxygenation injury. [Methods] H9C2 cardiomyocytes were cultivated in vitro and the model of hypoxia/reoxygenation injury was established. The cells were randomly divided into 3 groups: the control group, hypoxia/reoxygenation injury group and the geniposide pretreatment group. The contents of CK, LDH, MDA and the activity of SOD were evaluated by spectrophotometric method. The cell viability was measured with MTT. The apoptosis rate was detected by Annexin V-FITC/PI staining. [Results] Compared with the hypoxia/reoxygenation injury group, the geniposide pretreatment group can reduce the concentration of CK, LDH and MDA, improve the activity of SOD in myocardial tissue, improve the cell viability and reduce the apoptosis rate. [Conclusion] The geniposide has protective effects on hypoxia/reoxygenation injury, which are associated with scavenging free radical, enhancing the capacity of antioxidation and inhibiting cadioocyte apoptosis.关键词
栀子苷/心肌细胞/缺氧/复氧损伤Key words
geniposide/cardiomyocyte/hypoxia/reoxygenation injury分类
医药卫生引用本文复制引用
蔡智慧,尹丽,常全忠..栀子苷预处理对 H9C2心肌细胞缺氧/复氧损伤的影响及机制研究[J].天津中医药,2016,(2):111-113,3.基金项目
河南省教育厅高等学校重点科研项目（16A180031）。 （）
