中国组织工程研究Issue(52)：8384-8390,7.DOI:10.3969/j.issn.2095-4344.2015.52.004
钛微粒诱导破骨细胞活化中信号素7A siRNA的抑制作用
Inhibitory effect of Sema7A siRNA on osteoclast activation induced by titanium particles
摘要
Abstract
BACKGROUND:Sema7A is a kind of cel surface protein, which can promote the fusion of osteoclasts and the migration of osteoblast at the same time, affecting the dynamic balance of bone. OBJECTIVE:To investigate whether Sema7A siRNA has ainhibitory effect on the osteoclast activation in the process of osteolysis which induced by titanium particles. METHODS:The precursor osteoclasts with the concentration of 4×109 RESULTS AND CONCLUSION:At 7 days of culture, the expression levels of interleukin-1, interleukin-1β, tumor necrosis factor α, matrix metaloproteinase-9 and the receptor activator of nuclear factor-κB in the positive control, /L were seeded on 96-wel plates containing glass cover slips, and divided into four groups: blank control, positive control, experiment and negative control groups. The cel culture medium was added into the control group. 20 μL un-transfected siRNA supernatant was added into the positive control group. 20 μL transfected Sema7A siRNA supernatant was added into the experiment group. 20 μL transfected control siRNA supernatant was added into the negative control group. The supernatant was obtained through the co-culture between titanium particles solution and monocyte-macrophage cel line RAW264.7of mouse for 24 hours. siRNA was transfected into mononuclear macrophage cel lines RAW264.7 of mice. negative control and experiment groups were higher than those in the control group (P < 0.05). The expression level of each factor in the experiment group was lower than that in the positive control and negative control groups (P < 0.05). At 8 days of culture, the proliferation activity of osteoclasts and the number of positive cels stained by tartrate-resistant acid phosphatase in the positive control, negative control and experiment groups were higher than those in the control group (P < 0.05). The proliferation activity of osteoclasts and the number of positive cels stained by tartrate-resistant acid phosphatase in the experiment group were lower than those in the control and negative groups (P < 0.05). These results demonstrate that Sema7A siRNA has a certain inhibitory effect on the osteoclast activation induced by titanium particles.关键词
生物材料/骨生物材料/信号素7A siRNA/RANK/MMP-9/破骨细胞/人工关节/无菌性松动/骨溶解分类
医药卫生引用本文复制引用
丛宇,茹江英,赵云龙,俞磊,包倪荣,许斌,赵建宁..钛微粒诱导破骨细胞活化中信号素7A siRNA的抑制作用[J].中国组织工程研究,2015,(52):8384-8390,7.基金项目
南京军区医药卫生科研基金面上项目(14MS112)；江苏省临床医学科技专项资助(BL2012002)Funding:Nanjing Military Medical Scientific Research Program of China, No.14MS112 （14MS112）
the Clinical Medicine Science and Technology Program of Jiangsu Province, China, No. BL2012002 （）
