中国组织工程研究Issue(52)：8438-8443,6.DOI:10.3969/j.issn.2095-4344.2015.52.014
双层左旋聚乳酸静电纺织纳米纤维支架与人牙周膜细胞的生物相容性
Biocompatibility of double-layer poly(L-lactic acid) electrospun nanofiber scaffold with human periodontal ligament cells
孙文娟 1江浩顺 1黄南楠 1唐倩 1杨雨虹1
作者信息
- 1. 中山大学附属第三医院口腔科，广东省广州市 510630
- 折叠
摘要
Abstract
BACKGROUND:The morphological structure of nanofiber scaffold which fabricated by electrospinning technique is similar to the natural extracelular matrix, which provides a good microenvironment for cel growth and proliferation, and can also enhance cel adhesion, migration, proliferation and differentiation. OBJECTIVE: To observe the biocompatibility of double-layer poly(L-lactic acid) electrospun nanofiber scaffold and human periodontal ligament cels. METHODS: Electrospinning technique was used to prepare double layers poly(L-lactic acid) electrospun nanofiber scaffold. The toxicity of different concentrations of (100%, 75%, 50%, 25%) double-layer poly(L-lactic acid) electrospun nanofiber scaffold extracts to human periodontal ligament cels was evaluated by MTT assay. The double-layer poly(L-lactic acid) electrospun nanofiber scaffold was co-cultured with human periodontal ligament cels. The cel adhesive capacity in early stage was determined by MTT assay. The growth of cels on the scaffold was observed by scanning electron microscopy. RESULTS AND CONCLUSION: Different concentrations of double-layer poly(L-lactic acid) electrospun nanofiber scaffold extracts did not create any toxicity to human periodontal ligament cels. After co-culture for 2, 6, 24 hours, human periodontal ligament cels were poorly adherent onto the double-layer poly(L-lactic acid) electrospun nanofiber scaffold. After 7 days of co-culture, human periodontal ligament cels adhered wel on the loose surface of scaffold, maintained the original shape, stretched wel, and interconnected processes were observed; on the dense surface of the scaffold, multi-layer cels were observed. The cels showed fusiform or polygonal appearance and were connected together. These results demonstrate that the double-layer poly(L-lactic acid) electrospun nanofiber scaffold has good biocompatibility with human periodontal ligament cels.关键词
生物材料/材料相容性/牙周组织工程/左旋聚乳酸/支架材料/屏障膜/静电纺织/人牙周膜细胞分类
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孙文娟,江浩顺,黄南楠,唐倩,杨雨虹..双层左旋聚乳酸静电纺织纳米纤维支架与人牙周膜细胞的生物相容性[J].中国组织工程研究,2015,(52):8438-8443,6.
