中国当代儿科杂志Issue(2)：159-164,6.DOI:10.7499/j.issn.1008-8830.2016.02.012
解偶联蛋白2与脂多糖诱导脓毒症大鼠心肌线粒体损伤的关系
Correlation between uncoupling protein 2 expression and myocardial mitochondrial injury in rats with sepsis induced by lipopolysaccharide
摘要
Abstract
ObjectiveTo investigate the correlation between uncoupling protein 2 (UCP2) expression and myocardial mitochondria injury in rats with sepsis induced by lipopolysaccharide (LPS).MethodsThe rat model of sepsis was established through an intraperitoneal injection of LPS. Forty male Sprague-Dawley rats were randomly and equally divided into control group (an intraperitoneal injection of normal saline), sepsis 6 h group (LPS-6 h group), sepsis 12 h group (LPS-12 h group), sepsis 24 h group (LPS-24 h group), and sepsis 48 h group (LPS-48 h group). The serum and heart tissues were harvested at corresponding time points and myocardial mitochondria was extracted. The microplate reader was applied to measure creatine kinase (CK), creatine kinase-MB (CK-MB), and reactive oxygen species (ROS). Flow cytometry was applied to measure the degree of mitochondrial swelling and mitochondrial membrane potential (MMP). Western blot was used to measure the expression level of UCP2. Electron microscopy was applied to observe the morphological changes in heart tissues and myocardial mitochondria.ResultsCompared with the control group, the LPS groups had signiifcantly increased serum levels of CK, CK-MB, and myocardial ROS, as well as a signiifcantly increased degree of mitochondrial swelling (P<0.05), and these values reached their peaks at 24 hours after LPS injection. The LPS groups had a signiifcant decrease in MMP (P<0.05), which reached the lowest level at 24 hours after LPS injection. Western blot showed that the LPS groups had a signiifcant increase in the expression level of myocardial UCP2 compared with the control group (P<0.05), which reached its peak at 24 hours after LPS injection. The results of electron microscopy showed mitochondrial swelling, partial rupture of the mitochondrial membrane, and cavity formation in rats in the LPS groups. The most severe lesions occurred in the LPS-24 h group. In rats with LPS, the ROS level in the myocardial mitochondria and the degree of mitochondrial swelling were positively correlated with the expression level of UCP2 (r=0.796 and 0.893, respectively;P<0.05), while MMP was negatively correlated with the expression level of UCP2 (r=-0.903,P<0.05).ConclusionsIn the rat model of sepsis, the myocardium and myocardial mitochondria have obvious injuries, and the expression level of UCP2 is closely correlated with mitochondrial injury. Therefore, UCP2 might play an important role in myocardial mitochondrial injury in sepsis.关键词
脓毒症/心肌/线粒体/解偶联蛋白2/大鼠Key words
Sepsis/Myocardium/Mitochondria/Uncoupling protein 2/Rats引用本文复制引用
黄锦达,陈胜利,吕娟娟,刘翠,曾其毅..解偶联蛋白2与脂多糖诱导脓毒症大鼠心肌线粒体损伤的关系[J].中国当代儿科杂志,2016,(2):159-164,6.基金项目
国家自然科学基金（81272070）；广东省科技计划项目（2014A020212725）。 （）
