中国感染控制杂志Issue(1)：1-9,9.DOI:10.3969/j.issn.1671-9638.2016.01.001
肠致病性大肠埃希菌EPECDeng致病岛基因进化特点
Genomic evolution characteristics of pathogenicity islands of enteropatho-genic Escherichia coli Deng strain
摘要
Abstract
Objective To analyze the genomic evolution characteristics of pathogenicity islands (PAIs)in Deng strain of enteropathogenic Escherichia coli (E.coli,EPEC Deng).Methods EPEC Deng was isolated from infant stool specimen,serotypes were identified and antimicrobial susceptibility testing was performed;whole-genome se-quencing was performed by Illumina 2000 system,the locations of prophages(PPs)in the chromosome were detected using PHAST software,collinearity analysis was performed by MUMmer software,phylogenetic trees of homolo-gous gene were constructed in order to understand the evolutional rule of homology gene.PAIs prediction was per-formed using PAI finder software,the homologous evolutionary rule of PAIs core region(LEE)and core genes were clarified,genetic polymorphism was analyzed.Results The serotype of EPEC Deng strain was O119:H6,the strain was resistant to ciprofloxacin,levofloxacin,and ampicillin,but sensitive to other antimicrobial agents.The complete circular chromosome contained 5 025 482 bp with a GC content of 50.52 %,and the plasmid contained 207 564 bp with a GC content of 49.50%.A total of 17 PPs in the chromosomal genome were discovered,phyloge-netic trees analysis suggested that EPEC Deng strain was highly homologous with O26:H11 and O111 :H strains;PAIs and core genes were highly homologous with RDEC-1 and O26:H413/89-1 strains;genetic diversity analysis showed that the intimin (eae)and its receptor tir had high polymorphism,with the pi (π)value>0.10,the genes in type III secretion system was relatively stable.Conclusion The study clarified the genomic evolution characteris-tics of EPEC Deng genome and it’s PAIs,and is helpful for understanding genetic characteristics of native EPEC.关键词
肠致病性大肠埃希菌/高通量测序/全基因组序列/致病岛/核心基因/同源性Key words
enteropathogenic Escherichia coli/high-throughput sequencing/complete genome sequence/patho-genicity island/core gene/homology分类
医药卫生引用本文复制引用
陈重,邓启文,郑金鑫,杨唯枝,王红燕,姚伟明,邓向斌,李多云,刘晓军,余治健..肠致病性大肠埃希菌EPECDeng致病岛基因进化特点[J].中国感染控制杂志,2016,(1):1-9,9.基金项目
国家自然科学基金（81170370／H0312）；深圳市科创委项目（JCYJ20130402151227180）；深圳市卫人委项目（201203203、201203211、201302199、201303186、201401083、201401084、201402130、201402131、201402133）；深圳市医学重点学科经费资助 （）
