局解手术学杂志Issue(1)：11-16,6.DOI:10.11659/jjssx.07E015105
胃旁路术预防2型糖尿病的实验研究
Effect of Roux-en-Y gastric bypass postoperative on the blood glucose expression of type 2 diabetic rats
摘要
Abstract
Objective Recent studies have found that Roux-en-Y gastric bypass( RYGB) can inhibit the levels of blood glucose in type 2 diabetes,but its mechanism still remains unknown. In present study,we observed the effect of RYGB on insulin and GLP-1of type 2 diabetes mice. Methods The 12-week-old male SD rats were divided into four groups:group A ( no surgery,normal diet) ,Group B ( no surgery,DM diet+STZ injection) ,group C ( gastric bypass surgery+normal diet) ,group D ( gastric bypass surgery+DM diet+STZ injection) . One week after surgery rehabilitation,the diabetes model was built by STZ ( revulsant of the classical diabetes model) and high-fat-control diet. After four months,the changes of blood glucose,OGTT,body weight,food intake,water intake in each group were examined. Furthermore,the patho-logical changes of insulin and pancreatic were detected by HE staining. Meanwhile,the liver PEPCK gene and protein expression were detec-ted by using RT-PCR and Western blot. Results Four groups of rats all have significant changes in diet and weight. HE staining suggests the disseminated hyperemia and edema in pancreas and showed that islet has been severe damaged. Compared with no treatment normal diet,nor-mal rat+DM diet+STZ injection,has a markedly elevated blood glucose level 3 days later,insulin,OGTT,GLP,and ITT all have remarkable changes in different time periods,with a statistically significance (P<0. 05). Compared with the normal rat+DM diet+STZ injection group, RYGB + DM diet+ STZ injection group showed that these indicators of pancreas pathological changes significantly,glucose,insulin,OGTT, GLP,ITT all have significantly drop,as well as the gene and protein expression of PEPCK (P<0. 05). Conclusion RYGB can exert influ-ence on the change of insulin,OGTT,GLP,ITT and PEPCK in islets of type 2 diabetes rats,which may play a positive role in the further clini-cal applications.关键词
胃旁路术/2型糖尿病/预防/PEPCKKey words
Roux-en-Y gastric bypass/type 2 diabetic/prevention/PEPCK分类
医药卫生引用本文复制引用
谢杰斌,庞月珊,魏寿江,王崇树,唐锦..胃旁路术预防2型糖尿病的实验研究[J].局解手术学杂志,2016,(1):11-16,6.基金项目
四川省教育厅基金(13ZB0243) （13ZB0243）
川北医学院科研发展计划项目(CBY12-A-QN20) （CBY12-A-QN20）
