中国临床药理学杂志Issue(3)：241-244,4.DOI:10.13699/j.cnki.1001-6821.2016.03.015
噬菌体TM4 motif3蛋白制备及其复苏结核休眠菌的作用
Cloning and expression of motif3 of bacteriophage TM4 and its resuscitation activity on dormant mycobacterium
摘要
Abstract
Objective To investigate the activity of motif3 of mycobac-teriophage TM4 in resuscitating dormant mycobacterium tuberculosis on the basis of constructing bacteriophage TM 4 motif3 recombinant protein and the dormant model of mycobacterium tuberculosis respectively.Methods The objective motif3 fragment from bacteriophage TM 4 was amplified by polymerase chain reaction , and then cloned into the pET-28a(+) vector after purification and recovery.The recombinant plasmid was sequenced and expressed in E.coli BL21(DE3), then puri-fied and identified by Western blot.Different concentrations of motif 3 were added into dormant cells of mycobacterium tuberculosis respective-ly, then its resuscitation activity and the sterilization effect of isoniazid on resuscitated mycobacterium tuberculosis cells were detected.Results The recombinant motif3 which fused histidine residue was ob-tained.The objective protein was located at 12.65 ku.Additionally , the resuscitation activity was remarkable when the concentration of motif 3 was 1.1 ×10 4 pmol · L-1 .The resuscitated mycobacterium tuberculosis cells were killed by isoniazid.Conclusion The recombinant protein motif 3 had the resuscitation activity on the dormant mycobacterium tuberculosis.关键词
噬菌体TM4/基因表达/结核休眠菌/复苏Key words
bacteriophage TM4/gene expression/dormant mycobacterium tuberculosis/resuscitation分类
医药卫生引用本文复制引用
张莉,江莉莎,姚义勇,柳岩,郭述良..噬菌体TM4 motif3蛋白制备及其复苏结核休眠菌的作用[J].中国临床药理学杂志,2016,(3):241-244,4.基金项目
国家科技部“十二五”重大专项基金资助项目（2012ZX10003－009）；国家临床重点专科专项经费基金资助项目［卫办医政函2012（949号）］ （）
