中国临床药理学杂志Issue(3)：251-253,3.DOI:10.13699/j.cnki.1001-6821.2016.03.018
高效液相色谱荧光检测法测定人血清中环丙沙星浓度研究
Determination of ciprofloxacin in human serum by high performance liquid chromatography-fluorescence detector
戈梦佳 1刘洋 2李晓光1
作者信息
- 1. 北京大学 第三医院 药剂科，北京 100191
- 2. 北京大学 药学院，药事管理与临床药学系，北京 100191
- 折叠
摘要
Abstract
Objective To establish a method for determination of cipro-floxacin concentration in human serum by high performance liquid chro-matography-fluorescence detector method ( HPLC-FLD).Methods Ciprofloxacin and the internal standard ( IS) gatifloxacin were extracted from human serum after protein precipitation by 5%perchloric acid , then detected with fluorescence detector after excitation at 278 nm after sepa-ration by an Eclipse XDB -C18 (150 mm ×4.6 mm,5 μm).The mobile phase consisted of acetonitrile -phosphate ( pH 3 ) =15∶85 , with a flow rate of 1.0 mL· min-1 using gradient elution .Results Linear calibra-tion curves were obtained in the range of 0.05 -50.00 μg · mL-1 ( r>0.999 5 ).Intra-day RSD were in the ranges of 0.98%-2.63%and inter-day RSD were in the ranges of 1.87%-3.16%.Conclusion The method provides a sensitive , accurate , precise and reliable analy-tical procedure for clinical monitoring of ciprofloxacin in serum.关键词
环丙沙星/高效液相色谱荧光法/药物浓度/蛋白沉淀/药代动力学Key words
ciprofloxacin/HPLC -FLD/drug concentration/protein precipitation/pharmacokinetic分类
医药卫生引用本文复制引用
戈梦佳,刘洋,李晓光..高效液相色谱荧光检测法测定人血清中环丙沙星浓度研究[J].中国临床药理学杂志,2016,(3):251-253,3.
