中国临床药理学杂志Issue(4)：330-332,3.DOI:10.13699/j.cnki.1001-6821.2016.04.014
高效液相色谱－质谱联用法测定人血浆中氢吗啡酮－3－葡萄糖苷酸的质量浓度
HPLC-MS/MS for the determination of Hydromorphone-3-glucuronidecuronide in human plasma
陈学义 1郝光涛 1程龙妹 1张应福 1董瑞华 1王晓芳 1李媛媛 1刘泽源 1曲恒燕1
作者信息
- 1. 军事医学科学院 附属医院 临床药理室，北京 100071
- 折叠
摘要
Abstract
Objective To develop a HPLC -MS/MS method for the determination of hydromorphone -3 -glucuronide in human plasma.Methods The plasma was treated with solid -phase extraction.The column was XTerra®MS C18 column (2.1 mm ×50 mm,5 μm), the mobile phase consisted of 95% acetonitrile -5 mmol · L-1 ammonium bicarbonate with a gradient elution and the flow rate was 0.3 mL· min -1 , column temperature was 20 ℃ with positive ion scan and multiple reaction monitoring mode.The method was validated by investigation of the method specificity ,the standard curve and lower limit of quantification , precision and recovery , matrix effects and stability.Results Hydromorphone -3 -glucuronide had a good linear relationships in the range of 0.5-100.0 ng · mL-1 ( r>0.999 5 ) , the limit of quantification was 0.5 ng · mL-1.Intra -day and inter -day RSD were less than 15%, the average recovery were >60%, and stability was good.Conclusion The method was simple , rapid, sensitive and accurate , suitable for measuring the concentration of hydromorphone -3-glucuronide in human plasma.关键词
高效液相色谱-质谱联用法/氢吗啡酮-3-葡萄糖苷酸Key words
HPLC-MS/MS/hydromorphone-3-glucuronide分类
医药卫生引用本文复制引用
陈学义,郝光涛,程龙妹,张应福,董瑞华,王晓芳,李媛媛,刘泽源,曲恒燕..高效液相色谱－质谱联用法测定人血浆中氢吗啡酮－3－葡萄糖苷酸的质量浓度[J].中国临床药理学杂志,2016,(4):330-332,3.
