中国美容医学Issue(1)：37-40,4.
同种异体皮肤移植家兔注射重组人IL-10后血清IL-17家族水平的变化观察
Observation on serum IL-17 family level variation of rabbit treated with allogeneic skin grafting after injection of recombinational human IL-10
摘要
Abstract
Objective This study is in order to observe serum IL-17 family level variation of rabbits treated with allogeneic skin grafting after injection of recombinational human IL-10, and also in order to investigate immunoreaction inhibition mechanism of recombinational human IL-10.Methods 30 healthy new zealand white rabbits were randomly and averagely distributed into 6 groups, which included group A(recombinational human IL-10 low-dose group, 5μg /(kg.d)), group B(recombinational human IL-10 high-dose group, 10μg /(kg.d)), group C(cyclosporine low-dose group, 5mg /(kg.d)), group D(cyclosporine high-dose group, 10mg /(kg.d)) , group E(recombinational human IL-10 low-dose group, 5μg /(kg.d)& cyclosporine low-dose group, 5mg /(kg.d)) and group F(normal saline blank control group, 0.9% sodium chloride solution 1ml/d ), each group had 5 rabbits. These rabbits were continuously treated with drugs by intramuscular injection for 10days from 1 d before skin grafting operation,middle ear arterial blood was respectively collected for rabbits of all groups on 1d before operation, 1d, 4d, 1 week, 2 weeks,3weeks and 4 weeks after operation. Serum was separated. Enzyme linked immunosorbent assay was used for detection concentration of IL-17 family members(IL-17A-F).Results Concentration of IL-17A,IL-27(IL-17B/C/D),IL-17E(IL-25),IL-17F for rabbits after allogeneic skin grafting operation was higher than that before operation, concentration of IL-17A,IL-27(IL-17B/C/D),IL-17E(IL-25),IL-17F reached to the peak value on 2 weeks after operation, then concentration decreased. rhIL-10 could inhibit secretion of IL-17A,IL-27 and IL-17F. inhibiting effect was strengthened with the increase of rhIL-10 dose, rhIL-10 could promote secretion of IL-25,no obvious dose-effect relationship was found.Conclusion Serum IL-17 family level of rabbit treated with allogeneic skin grafting after injection of recombinational human IL-10 was obviously improved,inhibiting effect of rhIL-10 on immunoreaction might be related to IL-17 family mediated inflammatory reaction and secretion of inlfammatory factors.关键词
IL-17家族/重组人IL-10/家兔/同种异体/皮肤移植/免疫排斥反应Key words
IL-17 family/recombinational human IL-10/rabbit/allogeneic/skin grafting/immunological rejective reaction分类
生物科学引用本文复制引用
魏聪,刘远程,赵容..同种异体皮肤移植家兔注射重组人IL-10后血清IL-17家族水平的变化观察[J].中国美容医学,2016,(1):37-40,4.基金项目
人体营养素检测关键技术与产品开发主题项目（编号2014AA022304），本课题为国家高技术研究发展技术“863”计划；课题名称高血压患者同型半胱氨酸与肌红蛋白和游离脂肪酸相关性研究 （）
