中国免疫学杂志Issue(2)：189-192,4.DOI:10.3969/j.issn.1000-484X.2016.02.009
黄芪多糖对甲流HA2蛋白真核表达载体免疫效果影响的研究
Research of astragalus polysaccharides increasing immune effect of influenza A virus HA2 eukaryotic expression vector
摘要
Abstract
Objective:To study the astragalus polysaccharides ( APS) effect on immune induced by influenza A virus HA2 eu-karyotic expression vector.Methods: The HA2 encoded by the DNA vaccine vector was efficiently expressed in CHO cells, as determined by reverse transcription polymerase chain reaction ( RT-PCR) and fluorescence analysis.60 rats were divided into six groups randomly,which were immunized with normal saline,pEGFP-N1,pHA2/EGFP+different dose of APS by intramuscular injection.The control sera were collected before injection.After injected the 36th day, sera were collected to analyzing IFN-γ, IL-4 and IgG level.Results:IFN-γ,IL-4 and IgG level of pHA2/EGFP+mAPS group was different from that of pEGFP-N1 group or pHA2/EGFP+lAPS group( P<0.05 ).Conclusion: Middle dose of APS could increase immune induced by influenza A virus HA2 eukaryotic expression vector.关键词
流感病毒/血凝素/IFN-γ/IL-4Key words
Influenza A virus/Hemagglutinin/IFN-γ/IL-4分类
医药卫生引用本文复制引用
褚兆苹,戴军,吴淑慧,刘文泰,马志红,徐丙元,罗俊,曹刚,徐华洲,石玉娥..黄芪多糖对甲流HA2蛋白真核表达载体免疫效果影响的研究[J].中国免疫学杂志,2016,(2):189-192,4.基金项目
本文受河北中医学院博士科研基金（ BSZ2014002）和河北省卫生厅课题（20130139）资助。 （）
