农学学报2016，Vol.6Issue(1)：37-41,5.
4种杀菌剂对葡萄霜霉病菌的毒力及田间防效
Toxicity Test and Field Control Effect of 4 Different Fungicides on Grape Downy Mildew
摘要
Abstract
To investigate the biocontrol activity of Streptomyces corchorusii strain NF0919 and Bacillus subtilis DJ-6 WP to grape downy mildew, we determined the indoor toxicity of the supernatant of S. corchorusii strain NF0919, 1.0×1011 cfu/g B. subtilis DJ-6 WP, mancozeb and dimethomorph on Plasmopara viticola by using leaf disc method, respectively, and the field control tests were conducted. The results showed that the EC 50 values of the supernatant of strain NF0919, 1.0 × 1011cfu/g B. subtilis DJ-6 WP, mancozeb and dimethomorph were 96.2859, 86.6038, 69.9472 and 7.2636 μg/mL, respectively. The values of field control effect in preventive experiments on grape downy mildew on the 7th day after 2 times spraying with 20 times liquid of the supernatant of strain NF0919 and 1000 times liquid of 1.0 × 1011cfu/g B. subtilis DJ-6 WP were 71.55%and 70.71%, and the values of field control effect on the 14th day after 2 times spraying were 67.54%and 68.19%, respectively. The values of field control effect in curative experiments on the 7th day after 2 times spraying with 20 times liquid of the supernatant of strain NF0919 and 1000 times liquid of 1.0×1011cfu/g B. subtilis DJ-6 WP were 59.72%and 56.07%, the values of field control effect on 14th day after 2 times spraying were 56.88%and 57.46%, respectively. The field control effect of the two kinds of the tested biocontrol agents was equivalent. The protective effect between each of the tested biocontrol agents and 300 times liquid of 50%mancozeb WP had no significant difference, but there were significant differences of the efficacy between each of the tested biocontrol agents and 200 times liquid of 40% dimethomorph SC. In conclusion, the S. corchorusii strain NF0919 and B. subtilis DJ-6 WP had certain biocontrol potential to grape downy mildew and value of development.关键词
黄麻链霉菌NF0919菌株/枯草芽孢杆菌DJ-6/葡萄霜霉病/生防活性Key words
Streptomyces corchorusii Strain NF0919/Bacillus subtilis DJ-6/Grape Downy Mildew/Biocontrol Activity分类
农业科技引用本文复制引用
吉沐祥,吴祥,陈宏州,杨敬辉,庄义庆,姚克兵,李国平..4种杀菌剂对葡萄霜霉病菌的毒力及田间防效[J].农学学报,2016,6(1):37-41,5.基金项目
江苏省农业科技自主创新项目"江苏丘陵地区葡萄主要病害高效安全防控技术研究"[CX(14)2056] （14）
镇江市科技支撑计划项目"提高苏南丘陵地区应时鲜果生产市场竞争力的品种和技术的集成示范"(NY2013024). （NY2013024）
