中国全科医学Issue(5)：542-544,3.DOI:10.3969/j.issn.1007-9572.2016.05.011
心肌缺血再灌注后CXC趋化因子配体16水平的变化及调节机制
Change and Regulatory Mechanism of the Level of Plasma CXC - chemokine Ligand 16 After Myocardial Ischemia Reperfusion
摘要
Abstract
Objective To investigate the change and regulation mechanism of the level of plasma CXC - chemokine ligand 16(CXCL16). Methods We divided 30 New Zealand white rabbits into 3 groups:sham - operation group,cardiac ischemia/ reperfusion(I/ R)group and pyrrolidine dithiocarbamate(PDTC)group. The PDTC group was administrated with 20 mg/ kg PDTC by ear marginal vein injection,and 30 minutes later,the PDTC group and I/ R group were administrated with coronary artery ligation;1. 5 hours later,suture line was unbound;and the sham - operation group was only sutured without ligation. One hour after reperfusion,Thioflavine S was injected into atrium sinistrum,leaving no fluorescence staining in no reflow area. Ligation of left circumflex artery was made again at the primary site,and Evans blue was injected into atrium sinistrum again,with no blue stain area in ischemic area. After the animals were killed,ventricular slice was taken parallel to the atrioventricular groove,and the outlines of left ventricular wall,ischemic region and no reflow area were made manually on myocardial biopsy. During ligation,and 45 minutes,100 minutes and 150 minutes after ligation,ethylenediamine tetraacetic acid(EDTA)anticoagulative tube was employed to sample 5 ml blood from ear marginal vein,and double antibody sandwich enzyme immunoassay was used to determine CXCL16 level. Myocardial tissue staining results of left ventricular septal area, ischemic region and no flow area were obtained,and the dyeing positive rate of cell nucleus was used to reflect the expression of NF-κB p65. Results The area of myocardial ischemia and the no - flow area of PDTC group were both smaller than I/ R group (P ﹤ 0. 05). Different intervention measures were significantly different in the influence on the level of CXCL16 at different time points(P ﹤ 0. 05). In I/ R group,the level of CXCL16 at 100 minutes after ligation was higher(P ﹤ 0. 05)than that during ligation and 45 minutes after ligation. The three groups were significantly different in the dyeing positive rate of ischemic area and no - flow area(P ﹤ 0. 05);the ischemic area and no - flow area of the I/ R group were higher than sham - operation group and PDTC group in the dying positive rate of cell nucleus(P ﹤ 0. 05). Conclusion Myocardial ischemia reperfusion could induce the expression of CXCL16,and the inhibition of the activation of NF-κB could reduce the level of CXCL16 and reduce the myocardial ischemia area and no flow area.关键词
心肌再灌注/趋化因子 CXCL16/信号传导/NF-κBKey words
Myocardial reperfusion/Chemokine CXCL16/Signal transduction/NF-kappa B分类
医药卫生引用本文复制引用
曾敏,魏欣,李伟,蒙绪卿,符秀虹,陈积雄,王萍..心肌缺血再灌注后CXC趋化因子配体16水平的变化及调节机制[J].中国全科医学,2016,(5):542-544,3.基金项目
国家自然科学基金资助项目（81260043；81100153）；海南省应用技术研发与示范推广专项 （）
