中国全科医学Issue(5)：549-553,5.DOI:10.3969/j.issn.1007-9572.2016.05.013
膀胱尿路上皮癌标本中人微小核糖核酸3658与人源性长寿保障基因Ⅱ型基因表达的相关性及其临床意义
Correlation of miR-3658 and Homo Sapiens Longevity Assurance Homologue 2 Expression in Bladder Urothelial Carcinoma Samples and Its Clinical Significance
摘要
Abstract
Objective To investigate the expression differences and correlation of microRNA - 3658( miR-3658) and homo sapiens longevity assurance homologue 2(LASS2) in clinical bladder urothelial carcinoma samples. Methods We collected 96 patients with bladder urothelial carcinoma from the Departments of Urology in the Second Affiliated Hospital of Kunming Medical University and Yunnan Cancer Hospital from January 2014 to January 2015;bladder cancer tissues and paracarcinoma tissues were obtained from radical resection of bladder urothelial carcinoma. Hsa-miR-3658 and LASS2 mRNA expression in bladder cancer tissues and paracarcinoma tissues were detected by real - time fluorescence quantification PCR;LASS2 protein expression in bladder cancer tissues and paracarcinoma tissues was detected by immunohistochemistry( IHC). Results miR-3658 mRNA expression was higher in bladder cancer tissues than paracarcinoma tissues;and LASS2 mRNA expression was lower in bladder cancer tissues than paracarcinoma tissues;the two differences were both significant(t = 6. 46, 38. 12;P ﹤ 0. 01). miR-3658 mRNA and LASS2 mRNA expression were significantly different among patients with different recurrence status,pathological grade,infiltration depth,lymphatic metastasis,distal metastasis and TNM stages(P ﹤ 0. 05). LASS2 protein located in cytoplasm was brown or yellow - brown granules. The positive expression rate of LASS2 protein was lower in bladder cancer tissues than paracarcinoma tissues( χ2 = 15. 32,P ﹤ 0. 01). The positive expression rate of LASS2 protein was significantly different among patients with different recurrence status,pathological grades, infiltration depth, lymphatic metastasis and TNM stages( P ﹤ 0. 05). Pearson correlation analysis results showed LASS2 mRNA and miR-3658 mRNA expression were negatively correlated in bladder cancer tissues( r = - 0. 676,P ﹤ 0. 05). Conclusion miR-3658 expression is negatively correlated with LASS2 expression in bladder cancer tissues,and miR-3658 may participate in the occurrence and development of bladder cancer by regulating the LASS2 expression.关键词
膀胱肿瘤/微小核糖核酸/人源性长寿保障基因Ⅱ型/相关性Key words
Urinary bladder neoplasms/MicroRNA/LASS2/Correlation分类
医药卫生引用本文复制引用
梁敏,王海峰,王剑松,杨宏..膀胱尿路上皮癌标本中人微小核糖核酸3658与人源性长寿保障基因Ⅱ型基因表达的相关性及其临床意义[J].中国全科医学,2016,(5):549-553,5.基金项目
国家自然科学基金资助项目（81260374；81460384）；云南省教育厅基金资助项目（2014Z072）；云南省科技厅面上项目（2015FB196）；云南省科技厅-昆明医科大学联合专项（2014FA015；2014FZ031）；云南省卫生厅内设机构项目 （）
