中国全科医学Issue(6)：672-677,6.DOI:10.3969/j.issn.1007-9572.2016.06.010
重组人促红细胞生成素治疗肾间质纤维化的作用机制研究
Mechanism of Recombinant Human Erythropoietin in the Treatment of Renal Interstitial Fibrosis
摘要
Abstract
Objective To investigate the mechanism of recombinant human erythropoietin(rHuEPO)in the treatment of renal interstitial fibrosis(RIF). Methods From July 2014 to June 2015,divided the sampled human renal tubular epithelial cells(HK-2 cell)into 7 groups:E1 group( no irritants),E2 group(20 U/ ml rHuEPO),E3 group(5 mg/ ml purified albumin),E4 group(5 mg/ ml purified albumin + 5 U/ ml rHuEPO),E5 group( 5 mg/ ml purified albumin + 10 U/ ml rHuEPO),E6 group(5 mg/ ml purified albumin + 20 U/ ml rHuEPO),E7 group(5 mg/ ml purified albumin 30 min after 10μmol/ L Y27632). At 16,24,48 and 72 h,the number of proliferating cells of each group was detected by cell proliferation assay;RhoA and ROCK mRNA expression levels were detected by RT-PCR;α-SMA and E - cadherin levels were detected by cell immunofluorescence method;fiber connection protein(FN)expression level was examined by ELISA method. Results At 16 h,the 7 groups were not significantly different in the number of proliferating cells(P > 0. 05). At 24,48 and 72 h,the 7 groups were significantly different in the number of proliferating cells(P < 0. 05);E3 - E6 groups were higher than E1 group and E2 group;E4 - E6 groups were higher than E3 group;E7 group was lower than E3 group;E5 and E6 groups were higher than E4 group;E6 group was higher than E5 group(P < 0. 05). The 7 groups were significantly different in the expression level of RhoA mRNA(P < 0. 05);E3 - E6 groups were higher than E1 group and E2 group;E4 - E6 groups were lower than E3 group;E5 and E6 groups were lower than E4 group;E6 group was lower than E5 group(P < 0. 05). The 7 groups were significantly different in the expression level of ROCK mRNA(P < 0. 05);E3 - E6 groups were higher than E1 group and E2 group;E4 - E7 groups were lower than E3 group;E5 and E6 groups were lower than E4 group;E6 group was lower than E5 group(P < 0. 05). The 7 groups were significantly different in the expression level of α-SMA(P < 0. 05);E3 - E5 groups were higher than E1 group and E2 group;E4 - E7 groups were lower than E3 group;E5 - E6 groups were lower than E4 group;E6 group was lower than E5 group(P < 0. 05). The 7 groups were significantly different in the expression level of E - cadherin(P < 0. 05);E3 - E6 groups were lower than E1 group and E2 group;E4 - E7 groups were higher than E3 group;E5 - E6 groups were higher than E4 group;E6 group was higher than E5 group(P < 0. 05). The 7 groups were significantly different in the FN expression level(P< 0. 05);E3 - E5 groups were higher than E1 group and E2 group;E4 - E7 groups were lower than E3 group;E5 - E6 groups were lower than E4 group;E6 group was lower than E5 group(P < 0. 05). RhoA mRNA was positively correlated with ROCK mRNA in E3,E4,E5 and E6 group(P < 0. 05). Conclusion Purified albumin can induce the occurrence of EMT and further induce RIF. rHuEPO can inhibit the occurrence of RIF,and its inhibitory effect improves with the increase of rHuEPO level to some extent.关键词
肾疾病/细胞转分化/重组人促红细胞生成素/rho 相关激酶类/信号传导Key words
Kidney diseases/Cell transdifferentiation/Recombinant erythropoietin/rho - associated kinases/Signal transduction分类
医药卫生引用本文复制引用
江罗佳,秦晓华,黄翀,房向东,涂卫平..重组人促红细胞生成素治疗肾间质纤维化的作用机制研究[J].中国全科医学,2016,(6):672-677,6.基金项目
江西省教育厅科学技术研究项目 （）
