中国现代医学杂志Issue(2)：1-5,5.DOI:10.3969/j.issn.1005-8982.2016.02.001
Th22细胞和血清白细胞介素-22在胶原诱导的关节炎大鼠模型外周血的表达水平及其机制
Percentage of Th22 cell and serum IL22 level in peripheral blood：mechanism of collagen-induced arthritis in rat model
张保龙 1尤笑迎 1尹万乐 1田明波 1李达 1马利阁1
作者信息
- 1. 郑州人民医院 骨三科，河南 郑州 450000
- 折叠
摘要
Abstract
Objective To investigate the percentage of Th22 cells and the serum IL22 level in the rat model of collagen-induced rheumatoid arthritis (RA), and to study the mechanism of IL22 on RA. Methods After the model of collagen-induced RA was built, Th22 cells were detected by flow cytometry, the mRNA level of was detected by real-time PCR, and IL22 protein level was detected by ELISA. After the RA rats were injected with IL22 blocking antibody, the proliferation of rheumatoid arthritis fibroblast-like synovio-cytes (RAFLS) was observed by CCK-8, the mRNA levels of and were detected by real-time PCR, the level of STAT3 protein was observed by Western blot. Results The percentage of Th22 cells and the serum IL22 in the peripheral blood of RA rats were significantly higher than those in the control rats ( <0.01). After the RA rats were injected with IL22 blocking antibody, the expression of serum IL22 decreased ( <0.01) and the proliferation of RAFLS decreased ( <0.01), the levels of mRNA and STAT3 protein also significantly reduced ( <0.01). Conclusions Th22 cells are involved in the pathogenesis of RA in the rat model by stimulating STAT3 signaling pathway through IL22 secretion, which in turn promotes RAFLS pro-liferation.关键词
Th22细胞/IL- 22/关节炎/STAT3Key words
Th22 cell/IL22/arthritis/STAT3分类
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张保龙,尤笑迎,尹万乐,田明波,李达,马利阁..Th22细胞和血清白细胞介素-22在胶原诱导的关节炎大鼠模型外周血的表达水平及其机制[J].中国现代医学杂志,2016,(2):1-5,5.
