中国现代医学杂志Issue(2)：6-12,7.DOI:10.3969/j.issn.1005-8982.2016.02.002
从Tau蛋白过度磷酸化探讨2型糖尿病所致神经纤维化的机制*
Hyperphosphorylation of tau and mechanism of neural fibrosis of type 2 diabetes
摘要
Abstract
Objective To observe the effect of high blood sugar on cognitive ability of rats and the degree of phosphorylation of tau protein in the hippocampus, in order to investigate the mechanism of neural fibrosis in type 2 diabetes. Methods Two intervention factors were set in this study. One was type 2 diabetes model-ing method which included 3 levels, i.e. general diet, high-fat high-sugar high-protein diet, and high-fat high-sugar high-protein diet with intraperitoneal injection of small dose of Streptozotocin. The other was drug intervention in 2 levels: no disposal, and 4-week gavage of Rosiglitazone tablets at a dose of 3.0 mg/kg/d. Forty-eight SD rats were divided into 6 groups. Morris water maze was used to test the cognitive ability of the rats. Plasma glucose was determined by glucose oxidase method. The plasma insulin was determined by radioimmunoassay. The degree of insulin resistance was evaluated by HOMA-IR. The concentration of glutamic acid in hippocampus was determined by HPLC. The expressions of p-PHF1Ser396/404, p-AT8Ser199/202, p-12E8Ser262 in the hippocampus were determined by ELISA. Results Insulin resistance and type 2 diabetes could cause cog-nitive impairment. Rosiglitazone alleviated cognitive impairment. Insulin resistance and type 2 diabetes signifi-cantly increased the concentration of glutamic acid in the hippocampus. Rosiglitazone reduced the concentra-tion of glutamic acid. The insulin resistance and type 2 diabetes increased the phosphorylation of tau protein in the hippocampus. Rosiglitazone reduced the expression of phosphorylated tau protein in the hippocampus. Conclusions Insulin resistance and elevated blood sugar may be the causes of phosphorylation of tau protein in the hippocampus of type 2 diabetic rats. Rosiglitazone can mitigate this process.关键词
2型糖尿病/阿尔茨海默病/Tau蛋白/过度磷酸化/罗格列酮Key words
type 2 diabetes/Alzheimer's disease/tau protein/hyperphosphorylation/Rosiglitazone分类
医药卫生引用本文复制引用
马玲..从Tau蛋白过度磷酸化探讨2型糖尿病所致神经纤维化的机制*[J].中国现代医学杂志,2016,(2):6-12,7.基金项目
中国博士后科学基金面上项目 （）
