Chinese Journal of Modern MedicineIssue(2)：38-42,5.DOI:10.3969/j.issn.1005-8982.2016.02.008
原子力显微镜对雌激素受体表达的乳腺癌细胞形态特征的观察
Morphological characteristics of breast cancer cells with estrogen receptor expression under atomic force microscope
Abstract
Abstract
Objective To observe the surface morphological changes of breast cancer cell membrane with different expression of estrogen receptor (ER) under atomic force microscope (AFM), explore the mechanism of occurrence and development of breast cancer, as well as provide new ideas for early diagnosis of tumor on early sub-cellular level basis and possible evidence for advanced tumor therapy modalities. Methods The breast cancer cells were divided into two groups based on the level of ER expression: positive group and negative group. And then the surface morphology of cell membrane was observed under AFM. In both groups the characteristic morphological parameters were quantitatively measured for each cell, which included average roughness, mean peak height, average maximum depth and surface area difference. Then the results were statistically analyzed. Results There were significant differences between the two groups in cellular morphology. The surface of ER-positive cell membrane was rough and uneven: sharp tuberositas was similar to the awn of wheat; while in the ER-negative group, the tuberositas was crassitude and plump, just as the ditch. There were significant differences in the average roughness, mean peak height, average maximum depth and surface area difference between the cells of both groups ( <0.05). Conclusions The surface structure of breast cancer cell membrane varies with different functional state. This may provide us with an insight into the mechanism of actions of the cancer, its development process, and early diagnosis at pathological and clinical levels.关键词
原子力显微镜/乳腺癌/受体/细胞膜/形态特征Key words
atomic force microscope/breast cancer/estrogen receptor/cell membrane/morphological characteristic分类
医药卫生引用本文复制引用
姚文莲,纪小龙..原子力显微镜对雌激素受体表达的乳腺癌细胞形态特征的观察[J].中国现代医学杂志,2016,(2):38-42,5.
中国现代医学杂志
OA北大核心CSTPCD
1005-8982
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