中国现代医学杂志Issue(3)：9-13,5.DOI:10.3969/j.issn.1005-8982.2016.03.002
来曲唑配合高脂膳食诱导胰岛素抵抗多囊卵巢综合征大鼠代谢表型的实验研究*
Glucose and lipid metabolism in Letrozole and high-fat diet induced PCOS-IR rats
摘要
Abstract
Objective To investigate glucose and lipid metabolism in Letrozole and high-fat diet (HFD) induced polycystic ovary syndrome-insulin resistance (PCOS-IR) rats. Methods Twenty-four female SD rats of 3 weeks were randomly divided into 4 groups: control group, HFD group, PCOS group and PCOS-IR group. The rats in the HFD group and the PCOS-IR group were fed with high-fat diet for 12 weeks, while those of the control group and PCOS group were fed with normal diet. At the age of 6 weeks, the PCOS and PCOS-IR groups received Letrozole intragastric administration for 9 weeks. The changes in weight and ovarian mor-phology were observed; blood lipid, blood fasting plasma glucose (FPG), blood fasting insulin (FINS) and homeostasis model of assessment for insulin resistence index (HOMA-IR) were determined; and intraperitoneal glucose tolerance test (IPGTT) and insulin tolerance test were conducted in all the groups. Results Compared with the control and HFD groups, the PCOS and PCOS-IR groups showed polycystic ovaries with high inci-dence of atretic follicle and ovarian cyst together with decreased layers of granulosa cells and no corpora lutea. Compared with the control group, the PCOS-IR group exhibited increased weight ( =0.000) with higher total cholesterol concentration ( <0.05) and low-density lipoprotein concentration ( < 0.01), and increased AUC of IPGTT ( <0.05) and AUC of insulin tolerance ( <0.01). The PCOS-IR group also showed higher FINS concentration ( <0.01) and increased HOMA-IR ( <0.05). Conclusions Letrozole and high-fat diet can successfully induce typical metabolic phenotypes of insulin resistance in PCOS-IR rats.关键词
来曲唑/高脂饮食/糖脂代谢/胰岛素抵抗/多囊卵巢综合征Key words
Letrozole/high-fat diet/glucose and lipid metabolism/insulin resistance/polycystic ovary syndrome分类
医药卫生引用本文复制引用
林莺,张宏,黄鸣清,于志文,纪峰,黄黎珊,杨娟,许金榜..来曲唑配合高脂膳食诱导胰岛素抵抗多囊卵巢综合征大鼠代谢表型的实验研究*[J].中国现代医学杂志,2016,(3):9-13,5.基金项目
国家自然科学基金（No81302998）；福建省科技计划项目（No2015Y0002）；福建省中医药科研项目（Nowzfg201304）；福建省卫生计生委青年科研课题 （）
