中国现代医学杂志Issue(3)：39-44,6.DOI:10.3969/j.issn.1005-8982.2016.03.008
2014年深圳市流行的1型登革病毒分子溯源分析*
Molecular phylogenetic analysis of type 1 strains of dengue virus isolated in Shenzhen City during the 2014 dengue outbreak
摘要
Abstract
Objective To determine the envelope ( ) gene sequence of type 1 dengue virus (DENV-1) isolated in Shenzhen in 2014, and to understand the molecular virology characteristics of these strains as well as to explore their possible origin. Methods Thirty-five serum samples were collected from patients with dengue fever. Dengue virus was isolated by BHK-21 cells and the serotypes were detected by semi-nested polymerase chain reaction (PCR). The gene of the isolated strains was amplified by RT-PCR and sequenced. Homological and phylogenetic trees were also constructed based on the gene of isolated dengue virus strains. Results Totally 21 strains were isolated from 35 samples and identified as type 1 dengue virus. The complete coding region of genes from 6 strains of DENV-1 was all composed of 1,485 nucleotides which encoded 495 amino acids. Nucleotide sequence homology of 6 strains of DENV-1 was 100.0%identical, and their homology was very similar to the epidemic strains of DENV-1 virus in Shenzhen in 2010. The homology of the nucleotide sequence and the deduced amino acid sequence was 99.5% and 99.8% respectively. Compared with epidemic DENV-1 strains isolated from other countries, DENV-1 from Shenzhen in 2014 was close to the strains isolated in Singpore and Japan, and the highest homology of their genes and the deduced amino acids was 99.7% and 100.0%. The phylogenetic tree of genes indicated that the 6 strains of DENV-1 had the greater similarity with Shenzhen2010, Singpore2009 and Japan2004;and they all belonged to the genotype GⅠ. Conclusions DENV-1 strains from Shenzhen in 2014 belonged to the genotype GⅠ. They most likely originated from Southeast Asian countries. These epidemic dengue virus strains had been settled down in Shenzhen in 2010 and caused 2014 dengue fever outbreak.关键词
登革热/登革热病毒/基因/序列测定/系统进化树Key words
dengue fever/dengue virus/gene/sequencing/phylogenetic tree分类
医药卫生引用本文复制引用
马珍元,黄呈辉,张新枝,钟世良,田建华..2014年深圳市流行的1型登革病毒分子溯源分析*[J].中国现代医学杂志,2016,(3):39-44,6.基金项目
深圳市宝安区科技计划项目 （）
