中国药房Issue(4)：470-472,3.DOI:10.6039/j.issn.1001-0408.2016.04.12
滋肾育胎丸对电磁辐射大鼠卵巢组织氧化损伤和Nrf2蛋白表达的影响
Impacts of Zishenyutai Pills on the Ovarian Tissue Oxidative Damage and Nrf2 Protein Expression in Rats Exposed by Electromagnetic Radiation
摘要
Abstract
OBJECTIVE:To study the impacts of Zishenyutai pills on the ovarian tissue oxidative damage and Nrf2 protein ex-pression in rats exposed by electromagnetic radiation (EMR). METHODS:Accordiny to their weight,30 SD rats were randomly divided into normal group,radiated group and radiated-treated group with 10 rats in each group. Normal group were not radiated, and other 2 groups were exposed by 900 MHz radiation for 4 h every day;radiated-treated group was given Zishenyutai pills sus-pension [0.25 g intragstrically(medicinal materials)/ml] after radiation;normal group and radiated group were given constant vol-ume of normal saline intragastrically for consecutive 20 d. The rats were killed when they were at the estrum. The Nrf2 proteins ex-pression of ovary were detected by immunohistochemical method,the content of ovary MDA(malondialdehyde),and activity of to-tal superoxide dismutase (T-SOD) and superoxide dismutase (GSH-Px) were detected by biochemical method. RESULTS:Com-pared with the rats in normal group,the Nrf2 protein expression was higher in the rats of the radiated group;the content of ovary MDA was higher and activity of ovary T-SOD and GSH-Px were lower significantly(P<0.05). While compared with the rats in ra-diated group,the Nrf2 protein expression didn’t obviously change in the rats of the radiated-treated group(P>0.05),the content of ovary MDA was lower and activity of ovary T-SOD and GSH-Px were higher significantly (P<0.05). CONCLUSIONS:Zish-enyutai pills can prevent and cure ovary tissue oxidative injury induced by 900 MHz radiation,but have no effect on the expression of Nrf2 protein.关键词
电磁辐射/滋肾育胎丸/卵巢组织/核因子E2相关因子2/氧化损伤/大鼠Key words
Electromagnetic radiation/Zishenyutai pills/Ovary tissue/Nrf2/Oxidative stress/Rats分类
医药卫生引用本文复制引用
刘云肖,马惠荣,陈景伟,马雪莲,曹晓慧,陈杰,张国红..滋肾育胎丸对电磁辐射大鼠卵巢组织氧化损伤和Nrf2蛋白表达的影响[J].中国药房,2016,(4):470-472,3.基金项目
河北省科技计划项目（No.13277762D）；河北省高等学校科学技术研究项目 （）
