中国药房Issue(4)：479-481,3.DOI:10.6039/j.issn.1001-0408.2016.04.15
芹菜素在大鼠体内代谢产物的鉴定与分析
Identification and Analysis of Apigenin Metabolites in Rats
摘要
Abstract
OBJECTIVE:To clarify the bio-transformation form of apigenin in rats,and to speculate its possible metabolic path-way. METHODS:Rats were divided into blank group and medication group(apigenin 200 mg/kg,i.g.)with 6 rats in each group. Urine and feces samples were collected from 2 groups within 24 h after medication. After corresponding treatment,urine and feces samples were analyzed and detected by HPLC-IT-TOF-MSn under cation mode and anion mode. RESULTS:9 metabolites were iden-tified in urine sample of rats from medication group,i.e. 2,3-double bond reduction of apigenin (U1,U7,U8,U9),bonded to glucuronic acid(U2,U3,U4),bonded to sulphate(U5,U6,U7,U8,U9)and bonded to glucose(U2). 4 metabolites were iden-tified in feces sample of rats from medication group,i.e. 2,3-double bond reduction of apigenin(F3),bonded to glucuronic acid (F2)and bonded to glucose(F1). CONCLUSIONS:Apigenin mainly exists in form of prototype drug in rats. The reduction hap-pens on 2,3-double bond by the intestinal bacteria,and the product of apigenin boned to glucuronic acid or glucose can be formed when excreting in intestinal tract and rats in vivo,while the product of apigenin boned to sulphate can be formed only when excret-ing in rats in vivo.关键词
芹菜素/高效液相串联离子阱飞行时间多级质谱法/大鼠/尿液/粪便/代谢产物/鉴定/分析Key words
Apigenin/HPLC-IT-TOF-MSn/Rats/Urine/Feces/Metabolites/Identification/Analysis分类
医药卫生引用本文复制引用
张亚洲,王涛,邹树良,刘海林,晏晨..芹菜素在大鼠体内代谢产物的鉴定与分析[J].中国药房,2016,(4):479-481,3.基金项目
广西自然科学基金项目（No.2013GXNSFBA019187）；贵州理工学院高层次人才科研项目 （）
