中国现代中药Issue(1)：72-75,81,5.DOI:10.13313/j.issn.1673-4890.2016.1.008
人参总蛋白的酶解及氨基酸含量测定
Enzymolysis of Ginseng Total Proteins and Determination of Amino Acid Content
摘要
Abstract
Objective:The final active substances of ginseng proteins functioning in vivo were explored by comparing the component changes in ginseng proteins before and after enzymolysis.Methods:The ginseng proteins were hydrolyzed by pepsin,trypsin and double enzymes (pepsin and trypsin ), the protein and peptide content was detected by SDS-Polyacrylamide Gel Electrophoresis (SDS-PAGE),and the amino acid composition was analyzed by HPLC.Results:The results of SDS-PAGE showed that ginseng proteins were hydrolyzed into high concentration and low molecular weight (around 5000)proteins by pepsin,lower concentration proteins by trypsin,and proteins by double enzymes similar to those of pepsin hydrolysis.The results of HPLC showed that the amino acids in ginseng proteins decreased significantly after pepsin,trypsin and double enzymes hydrolysis.Conclusion:Ginseng proteins were converted into low molecular weight proteins and a small amount of amino acids after enzymolysis.关键词
人参蛋白/胃蛋白酶/胰蛋白酶/氨基酸Key words
Ginseng protein/pepsin/trypsin/amino acid引用本文复制引用
李红艳,张江华,张惠,陶震宇,杨静娴,康廷国..人参总蛋白的酶解及氨基酸含量测定[J].中国现代中药,2016,(1):72-75,81,5.基金项目
中国博士后科学基金项目 （）
