北京中医药大学学报Issue(1)：10-15,6.DOI:10.3969/j.issn.1006-2157.2016.01.002
利湿活血方对高尿酸血症模型大鼠血管内皮功能的影响∗
Lishi Huoxue formula to protect the vascular endothelial cells against hy-peruricemia in rats
摘要
Abstract
Objective To explore the effects and mechanism of Lishi Huoxue ( damp-dispersing and blood-activating) formula and two decomposed recipes Chinese herbal compound, on the vascular endo-thelial cells by detecting the serum levels of functional indicators of vascular endothelial cells and the aor-tic morphology of the rat model of hyperuricemia. Methods Male SD rats were randomly divided into seven groups:normal group, model group, benzbromarone group, Simiao Pill group, Lishi Huoxue for-mula (LSHX) group, decomposed recipe I for clearing heat and damp (recipe I) group, decomposed recipe Ⅱfor activating blood and resolving stasis ( recipeⅡ) group. The rat model of hyperuricemia was established by intragastrical ( i. g. ) administration of yeaut and adenine. After successful modeling, rats of each medication group were administered i. g. with corresponding drugs, while normal group and mod-el group were i. g. distilled water for consecutive 14 days. Then the serum contents of uric acid ( UA) and nitric oxide (NO) were detected by ultraviolet-visible spectroscopy, and endothelin (ET-1), throm-boxane B2 ( TXB2 ) and 6-keto-PGF1αwere measured by radioimmunoassay. Besides, thoracoabdominal a-orta of all rats were dissected and stained using HE and Masson. Results Compared with normal group, the serum level of UA, ET-1 and TXB2 increased significantly in model group(P<0. 05) and level of NO and 6-keto-PGF1αdecreased(P<0. 05). Compared with the model group, the serum contents of UA, ET-1 and TXB2 showed significantly decreasing in LSHX group(P<0. 05),while in recipe I group UA and ET-1 decreased(P<0. 05)and serum contents of NO, 6-keto-PGF1α in LSHX group, recipe ⅠandⅡ groups increased (P<0. 05). Furthermore, compared with LSHX group, the levels of TXB2 in both recipe groups and ET-1 in recipe Ⅱ group showed on statistical difference. Although LSHX and the two partial prescriptions ( recipe I, II) protected against the injury of aorta, the whole prescription ( LSHX) was more effective than the two decomposed recipes. Conclusion LSHX formula could reduce the serum UA, and protect vascular endothelium and the aorta of rats with hyperuricemia.关键词
高尿酸血症/尿酸/一氧化氮/内皮素/血栓烷素B2/6酮前列腺素/主动脉形态/利湿活血方/大鼠Key words
hyperuricemia/uric acid/nitric oxide/endothelin/thromboxane B2/6-keto-PGF1α/aortic morphology/Lishi Huoxue formula/rats分类
医药卫生引用本文复制引用
刘畅,王淳,郑媛,王林元,李伟,张建军..利湿活血方对高尿酸血症模型大鼠血管内皮功能的影响∗[J].北京中医药大学学报,2016,(1):10-15,6.基金项目
国家自然科学基金资助项目(No.81273632) （No.81273632）
