北京中医药大学学报Issue(1)：26-29,4.DOI:10.3969/j.issn.1006-2157.2016.01.005
黄芪注射液对视神经牵拉伤大鼠NGF、TrkA表达的影响∗
Astragalus Injection mediates the expressions of NGF and TrkA in retina tissue of rats optic nerve stretch injury model
摘要
Abstract
Objective To observe the effects of Astragalus Injection ( AI ) on the expressions of nerve growth factor(NGF) and tyrosine kinase receptor A(TrkA)in retinal tissue of rat model of optic nerve injury by stretch. Methods Altogether 48 healthy male Wistar rats were randomly divided into three groups:sham group, model group and AI group ( n=16 each ) . The rat model of optic nerve injury was induced by horizontal stretching the optic nerve of left eye by tensiometer with 0. 10 N for 20 seconds, and the rats of sham group were only exposed optic nerves, without undergoing stretching. After success-ful modeling, the rats of AI group were injected intraperitoneally ( i. p. ) AI and the those of model group were i. p. normal saline for consecutive 14 days. The retinal tissues of all rats were dissected out of the eye balls and the expressions of NGF and TrkA were detected by Western Blot. Results Compared with the sham group, the expression levels of NGF and TrkA in the model group decreased significantly( P<0. 05). After 14-day-treatment, the expressions of NGF and TrkA of retinal tissues increased in AI group compared with the model group(P<0. 05). Conclusion The protection of AI against optic nerve injury was proved by upregulating of the expressions of NGF and TrkA in the rat retinal tissue.关键词
视神经损伤/黄芪注射液/神经生长因子/酪氨酸激酶受体A/大鼠Key words
optic nerve injury/Astragalus Injection/nerve growth factor/tyrosine kinase receptor A/rats分类
医药卫生引用本文复制引用
谷新怡,刘爱伟,苏艳,闫晓玲,吴鲁华,夏燕婷,赵朋波,周剑..黄芪注射液对视神经牵拉伤大鼠NGF、TrkA表达的影响∗[J].北京中医药大学学报,2016,(1):26-29,4.基金项目
国家自然科学基金资助项目(No.81173307) （No.81173307）
