北京中医药大学学报Issue(1)：30-34,5.DOI:10.3969/j.issn.1006-2157.2016.01.006
滋阴息风方对自发性高血压大鼠肾脏JAK/STAT信号通路的影响∗
Influences of Ziyin Xifeng Fang on the JAK/STAT signal pathway in the kidney of spontaneously hypertensive rats
摘要
Abstract
Objective To observe the effects of Ziyin Xifeng Fang ( ZYXFF) , a Chinese medicine com-pound formulated by the method of nourishing yin and extinguishing wind, on the blood pressure and renal JAK/STAT signal pathway in the rat model of spontaneously hypertension ( SH) . Methods 40 SH rats were randomly divided into four groups ( n=10 each):model group, low dose of ZYXFF( low-dose) group, high dose of ZYXFF ( high-dose) group and compound apocynum group. Another 10 SKY rats with the same background were included in the control group. The rats of three treatment groups were ad-ministered intragastrically ( i. g. ) proper drugs, and those of control group and model group were i. g. sa-line for consecutive 42 days. The systolic blood pressure ( BP) was measured by using small animal non-invasive blood pressure meter at Day 0, 7, 14, 21, 28, 35 and Day 42. Two hours after i. g. at the last day of medication, all the rats were sacrificed. The serum contents of creatinin ( Cr) , blood urea nitrogen ( BUN) , and uric acid ( UA) were determined. The serum contents of IL-6 and TNF-αwere detected by ELISA assay, and the protein expression of renal p-JAK1 and p-STAT1 were measured by Western blot. Results Compared with control group, BP, Cr, BUN, UA, IL-6 and TNF-α increased significantly in model group, and the all indexes of the rats of the three medication groups decreased remarkably ( P<0. 05 or P<0 . 01 ) , especially the effects of high dose of ZYXFF and compound apocynum were stronger than those of low dose ZYXFF. Compared with control group, in model group rats renal p-JAK1, p-STAT1 protein expression significantly increased ( P<0 . 01 ) . The protein expressions of p-JAK1 and p-STAT1 decreased in the three medication groups compared with the model group ( P <0 . 05 or P <0 . 01 ) . Conclusion The mechanism of ZYXFF lowering blood pressure and attenuating the renal function impairment should be by the way of inhibiting the activation of JAK/STAT signal pathway.关键词
滋阴息风方/高血压/肾脏损伤/JAK/STAT信号通路/大鼠Key words
Ziyin Xifeng Fang/hypertension/kidney damage/JAK/STAT signal pathway/rats分类
医药卫生引用本文复制引用
王俊岩,张林,贾连群,陈文娜,孙宏伟,邵楚茜,杨关林..滋阴息风方对自发性高血压大鼠肾脏JAK/STAT信号通路的影响∗[J].北京中医药大学学报,2016,(1):30-34,5.基金项目
国家自然科学基金资助项目(No.81001493),辽宁省高等学校杰出青年学者成长计划项目(No. LJQ2014103),辽宁省高等学校优秀人才支持计划项目(No. LR2015041),辽宁中医药大学大学生创新创业计划项目(No.2014101620004) （No.81001493）
