北京中医药大学学报Issue(1)：35-39,5.DOI:10.3969/j.issn.1006-2157.2016.01.007
大黄酚对原代培养小鼠星形胶质细胞谷氨酸代谢的影响∗
Effects of chrysophanol on glutamate metabolism of primarily cultured strocytes in mice
摘要
Abstract
Objective To study the effects of different concentration of chrysophanol on the activity and glutamate metabolism of primarily cultured astrocytes in vitro. Methods Primarily cultured mice astro-cytes were treated with chrysophanol at concentration of 10 , 20 , 40 , 60 , 80 , 100 , 200 , and 250 mg/L ( chry 1~8 group) for 1 d, 2 d, and 3 d. The cell activities were measured by MTT method, mRNA ex-pressions of glutamate/aspartate transporter (GLAST) and glutamate acid transporter 1(GLT-1) were de-tected by using RT-PCR, and the activities of glutamine synthetase ( GS ) and glutathione peroxidase ( GSH-Px ) were also determined. Results Time-dependence and concentration-dependence of the effects of chrysophanol showed on the following: activity of astrocytes inhibited, mRNA expressions of GLAST and GLT-1 upregulated, and the activities of GSH-Px and GS increased. However, the effect of chrysophanol on extra-cellular concentration of glutamate didn’ t depend on the number or activity of the cells. Conclusion Chrysophanol inhibited the activity of astrocytes in mice, and its effect on glutamate metabolism was concentration-and time-dependent.关键词
大黄酚/星形胶质细胞/谷氨酸/天门冬氨酸转运体/谷氨酸转运体1/谷胱甘肽过氧化物酶/谷氨酰胺合成酶/小鼠Key words
chrysophanol/astrocyte/glutamate/aspartate transporter/glutamate acid transporter 1/glu-tathione peroxidase/glutamine synthetase/mice分类
医药卫生引用本文复制引用
薛占霞,高永山,武海霞,郝军荣,沈丽霞..大黄酚对原代培养小鼠星形胶质细胞谷氨酸代谢的影响∗[J].北京中医药大学学报,2016,(1):35-39,5.基金项目
河北省教育厅优秀青年基金资助项目(No. Y2012002),河北省教育厅重点项目(No. ZD2015131) （No. Y2012002）
