北京中医药大学学报Issue(1)：40-45,6.DOI:10.3969/j.issn.1006-2157.2016.01.008
红鹤方制剂HPLC指纹图谱研究∗
HPLC fingerprint study of Honghe Fang preparation
摘要
Abstract
Objective To establish the HPLC fingerprint of new preparation of Honghe Fang, a Chinese medicine compound, and to study the correlation of the preparation , effective components, and the fin-gerprint of raw herbal materials, so as to underlie its quality control. Methods The effective compo-nents were separated by HPLC with Inertsil ODS-3 column(5 μm, 4. 6 mm × 250 mm)in a gradient elu-tion of acetonitrile and water with 0. 01 H3 PO4 as the mobile phase at the flow rate of 1 mL/min and tem-perature of 25℃ and detection wavelength of 338 nm for 125 min. Then the common peaks were analyzed and identified by the alignment of HPLC fingerprint of Honghe Fang, effective components of raw herbal medicines, raw herbal medicines and negative control preparation. Results Altogether 28 common peaks separated completely from the 10 batches of Honghe Fang were confirmed by using the software“Similarity Evaluation System for Chromatographic Fingerprint of TCMs ( Version 2004 A )”, and of which six peaks were identified. The similarity based on the information of common peaks were all above 0. 96, a high similarity index. And each common peak could be identified in HPLC chromatograms of ef-fective components and raw herbal medicines. Conclusion This method proved to be simple, accurate and repeatable, could be applied in quality control of Honghe Fang.关键词
红鹤方制剂/有效组分/指纹图谱/质量控制Key words
Honghe Fang preparation/effective components/fingerprint/quality control分类
医药卫生引用本文复制引用
王晴,王英姿,李友林,倪健,张秀婷,李凤英,张春泥..红鹤方制剂HPLC指纹图谱研究∗[J].北京中医药大学学报,2016,(1):40-45,6.基金项目
国家科技重大专项课题(No.2012ZX09103201-044),北京中医药大学协同创新建设计划(No.2013-XTCX-03),北京中医药大学校级研究生自主课题(No.2015-JYB-XS076) （No.2012ZX09103201-044）
