北京中医药大学学报Issue(1)：46-50,5.DOI:10.3969/j.issn.1006-2157.2016.01.009
人肠内菌对藏药余甘子鞣质部位代谢的研究
Effects of human gut microbiota in vitro on transformation of the total tannins content of Phyllanthus emblical L
摘要
Abstract
Objective To study in vitro the effect of human intestinal bacteria on the transformation of to-tal tannins extracted from emblic leafflower ( Yuganzi, Phyllanthus emblica L. ) , a usually used Tibetan herbal medicine. Methods Based on the earlier stage work of the extraction of total tannins from Phyl-lanthus emblica L. , activated human gut microbiota was anaerobically incubated in the culture medium GAM containing the total tannins at 37℃ for 72 hours. After centrifugation and protein precipitation, the characteristics of the metabolites were determined by using HPLC/MSn and the contents of each compo-nent determined were measured by using HPLC-UV, on which the time-content curve was drawn. Results Total 13 components were determined, including four metabolites ( pyrogallic acid, methyl-de-rivative of gallic, urolithin B, and urolithin A) , four prototype components ( gallic acid, corilagin, gal-loyl glucose, and digalloyl glucose) and five components to be determined. The accumulated contents of gallic acid and ellagic acid were the highest at Hour 12 , 10-fold higher than that of the beginning; the accumulated content of corilagin was the highest at Hour 6 with twice higher of the beginning. Conclusion After the total tannins from Phyllanthus emblica L. were transformed by human gut microbi-ota in vitro, certain bioactive prototype components remained and antitumor components were found.关键词
余甘子/鞣质部位/人肠内细菌/代谢Key words
Phyllanthus emblica L./total tannins/human gut microbiota/metabolism分类
医药卫生引用本文复制引用
杨光辉,张兰珍#,吴玲芳,张晓雪,朱丹,邵岩岩,毛鑫,梁文仪,陈文静,石任兵..人肠内菌对藏药余甘子鞣质部位代谢的研究[J].北京中医药大学学报,2016,(1):46-50,5.基金项目
国家自然科学基金资助项目(No.81274187),北京中医药大学科研创新团队项目(No.2011-CXTD-12),北京中医药大学中青年教师资助项目(No.2009TYB22-JS027) （No.81274187）
