北京中医药大学学报Issue(1)：51-54,4.DOI:10.3969/j.issn.1006-2157.2016.01.010
间接竞争ELISA法测定不同厂家葛根芩连片中黄芩苷的含量∗
Indirect competitive enzyme-linked immunosorbent assay for determina-tion of baicalin in Gegen Qinlian Tablet produced by seven manufactur-ers
摘要
Abstract
Objective To determine the contents of baicalin in Gegen Qinlian Tablet produced by seven manufacturers, a Chinese patent drug used widely in acute gastroenteritis, virus diarrhea, and bacillary dysentery, by using indirect competitive enzyme-linked immunosorbent assay ( ic-ELISA ) . Methods The ic-ELISA method was established by using anti-baicalin monoclonal antibody ( anti-BA MAb) , in which the linear relation, accuracy and the recovery rate were measured. Based on the ic-ELISA method, the contents of baicalin in Gegen Qinlian Tablet from different manufacturers were deter-mined. Results The linear range of the ic-ELISA method established was from 16. 157 to 1 280 mg/L, the relative standard deviation (RSD) was≤5. 1% in the intra-assay, and≤9. 0% in the inter-assay, with the average recovery rate of 107 . 1% ( RSD=4 . 9%) . The baicalin contents in Gegen Qinlian Tab-let from seven manufacturers ranged from 4. 45 to 13. 41 mg per tablet. Conclusion The ic-ELISA method for the baicalin determination was convenient, accurate, and repeatable, which could be applied to the quality control of traditional Chinese herbal compound containing baicalin.关键词
间接竞争ELISA法/葛根芩连片/黄芩苷/含量测定Key words
indirect competitive enzyme-linked immunosorbent assay method/Gegen Qinlian tablet/ba-icalin/content determination分类
医药卫生引用本文复制引用
刘姝晨,屈保平,孔慧,单文超,成金俊,曾文浩,屈会化,王庆国,赵琰..间接竞争ELISA法测定不同厂家葛根芩连片中黄芩苷的含量∗[J].北京中医药大学学报,2016,(1):51-54,4.基金项目
国家自然科学基金资助项目(No.8147338) （No.8147338）
