北京中医药大学学报Issue(1)：61-66,6.DOI:10.3969/j.issn.1006-2157.2016.01.012
高山大黄的质量控制研究∗
Establishment of quality control of Rheum nobile
摘要
Abstract
Objective To explore the method to control the quality of Rheum nobile. , a widely used Tibet-an herbal medicine. Methods The herb powder of ten batches of Rheum nobile was identified by thin layer chromatography ( TLC) with piceatannol-4′-O-β-D-glucopyranoside as the reference substance, and the moisture content, total ash and acid-insoluble ash were detected in accordance with Appendix IX of Chinese Pharmacopoeia 2010 Edition. The contents of piceatannol-4′-O-β-D-glucopyranoside and res-veratrol-4′-O-β-D-glucopyranoside were determined by using HPLC. Results The spots of piceatannol-4′-O-β-D-glucopyranoside of ten batches of Rheum nobile were clear and well separated. The range of moisture content was from 7 . 46% to 9 . 14%, and that of total ash was 2 . 72% to 8 . 75%, and that of acid-insoluble ash 0 . 50% to 2 . 51%. The content of piceatannol-4′-O-β-D-glucopyranoside was showed a good linear relationship at the range of 1 . 768 to 70 . 720 mg/L ( r=0 . 999 8 ) and the recovery rate was 101. 11% ( RSD =0. 89%), by parallel, that of resveratrol-4′-O-β-D-glucopyranoside was showed a good linear relationship at the range from 0 . 416 to 16 . 640 mg/L ( r=0 . 999 6 ) and the recovery rate was 99. 68% (RSD=2. 54%). Conclusion The methods of TLC and HPLC are simple, accurate and re-peatable, which could be the option of the quality control of Rheum nobile.关键词
高山大黄/薄层鉴别/高效液相色谱法/含量测定Key words
Rheum nobile/TLC identifation/HPLC/content determination分类
医药卫生引用本文复制引用
宋盼红,费烨,曾祖平,彭冰,闫兴丽,陈静,多杰仁青,高增平..高山大黄的质量控制研究∗[J].北京中医药大学学报,2016,(1):61-66,6.基金项目
北京中医药大学自主选题(藏医药类)资助项目(No.2013-zyy-02) （藏医药类）
