北京中医药大学学报Issue(1)：67-72,6.DOI:10.3969/j.issn.1006-2157.2016.01.013
基于抗炎药效实验优选苦豆子提取工艺∗
Optimization extraction of Sophora alopecurcurodies based on the anti-in-flammatory effect experiment combined with orthogonal test
摘要
Abstract
Objective To optimize the procedure of extracting components from Sophora alopecurcurodies ( Kudouzi) . Methods After external use of the extracts of Kudouzi by four different extraction proce-dures on the footpad inflammation rat model induced by carrageenan, the swelling rate was observed as the therapeutic index. The contents of oxysop hocarpine and oxymatrine of extracts from Kudouzi, as the two chemical indexes, were measured by using HPLC. The extraction procedure was screened by thera-peutic index combined with chemical indexes. Then the parameters of the procedure screened were opti-mized by orthogonal test. Results All the extracts inhibited footpad swelling in rats, and the effect of the ethanol extract was the strongest, and in which the contents of oxysop hocarpine and oxymatrine were the highest. The footpad swelling rate was negatively correlated with the contents of the two chemical in-dexes. The optimized extraction procedure was obtained with 10 times of 80%( V/V) alcohol, 4 hours at 50℃. Conclusion The extraction procedure of Kudouzi was stable and feasible, and the contents of ac-tive components were positively correlated with their effects.关键词
苦豆子/提取方法/药理指标/化学成分/正交试验Key words
Sophora alopecurcurodies/extraction procedure/therapeutic indexes/chemical components/orthogonal test分类
医药卫生引用本文复制引用
李辉,马书伟,刘艳,茹庆国,刘葭,陈岩岩,吴清..基于抗炎药效实验优选苦豆子提取工艺∗[J].北京中医药大学学报,2016,(1):67-72,6.基金项目
国家自然科学基金资助项目( No.81473365),北京市自然科学基金资助项目( No.7132127),北京中医药大学创新团队发展计划项目(No.20011-CXTD-13) （ No.81473365）
