北京中医药大学学报Issue(2)：101-105,5.DOI:10.3969/j.issn.1006-2157.2016.02.003
慢性心力衰竭血瘀证患者血浆代谢物研究∗
Research on plasma metabolites of chronic heart failure patients with blood stasis pattern
摘要
Abstract
Objective To study the changes of metabolites in chronic heart failure ( CHF ) patients of blood stasis pattern by applying nuclear magnetic resonance ( NMR) . Methods Demographic data were collected from CHF patients of blood stasis pattern using cross-section survey. Their blood samples were also obtained and then magnetic resonance spectrum with pattern recognition techniques were used for in-tegral calculation and normalization based on chemical shift. All data were imputed into SIMCA-P soft-ware and the results were analyzed by using orthogonal projections to latent structures-discriminant analy-sis ( OPLS-DA) model. Results The OPLS-DA analysis of plasma samples could differentiate and pre-dict blood stasis pattern. Increased levels of lactate, alanine, and pyruvic acid with decreased levels of histidine, glycine, valineas, and other metabolites constitute the metabolomics profile of blood stasis pat-tern. Conclusion This study showed a profile of altered metabolites related to energy utilization and fat-ty acid metabolism in plasma samples of CHF patients with blood stasis pattern, which could offer a new approach to the diagnosis and treatment of CHF.关键词
代谢组学/慢性心衰/血瘀证/正交偏最小二乘判别分析法Key words
nuclear magnetic resonance/metabonomics/chronic heart fallure/orthogonal projections to latent structures-discriminant analysis分类
医药卫生引用本文复制引用
王娟,赵慧辉,陈建新,邓冬,郭淑贞,李中峰,罗良涛,王伟..慢性心力衰竭血瘀证患者血浆代谢物研究∗[J].北京中医药大学学报,2016,(2):101-105,5.基金项目
国家自然科学基金资助项目(No.81302914),国家中医药管理局行业专项(No.2008007) （No.81302914）
