北京中医药大学学报Issue(2)：113-118,6.DOI:10.3969/j.issn.1006-2157.2016.02.005
益气活血化痰中药复方对大鼠胚胎神经干细胞生长分化增殖的影响机制研究∗
Effects of Yiqi Huoxue Huatan Formula on proliferation and differentia-tion of rat embryonic neural stem cells
摘要
Abstract
Objective To study the effects of Yiqi Huoxue Huatan ( qi-replenishing blood-activating phlegm-resolving) Formula on proliferation and differentiation of rat embryonic neural stem cells ( NSC) . Methods The rat NSC were divided into five groups, including blank serum group, drug serum group, blank serum + LY294002 group, drug serum + LY294002 group, and culture medium control group. The effects of drug serum on cell proliferation and differentiation were evaluated by cell culturing, serum pharmacology, immunofluorescence techniques. Morphological changes were observed by phase contrast microscope. Nestin, neuron-specific enolase ( NES ) and glial fibrillary acidic protein ( GFAP ) were measured by using immunofluorescence staining. Results Morphologically, drug serum was non-toxic to neural stem cells and promoted the differentiation of neural stem cells. Immunofluorescence staining showed that nestin expression increased within 24 h yet decreased significantly after 72 h incubation. NES and GFAP positive cells increased significantly in the drug serum group, compared with control group. Conclusion The serum from the rats treated with Yiqi Huoxue Huatan Formula has significant effects on differentiation and proliferationof NSC.关键词
益气活血化痰中药/神经干细胞/生长/分化/增殖/大鼠Key words
Yiqi Huoxue Huatan Formula/neural stem cells/growth/differentiation/proliferation/rats分类
医药卫生引用本文复制引用
郜峦,王键,程发峰,唐巍..益气活血化痰中药复方对大鼠胚胎神经干细胞生长分化增殖的影响机制研究∗[J].北京中医药大学学报,2016,(2):113-118,6.基金项目
国家自然科学基金项目(No．81102547),国家科技支撑计划(No.2013CB532001) （No．81102547）
