北京中医药大学学报Issue(2)：122-127,6.DOI:10.3969/j.issn.1006-2157.2016.02.007
Toll样受体8基因rs3764880多态性与汉族男性缺血性中风的关联性研究
Toll-like receptor-8 gene rs3764880 polymorphism and ischemic stroke risk in patients of Han Chinese males
摘要
Abstract
Objective To explore the association between Toll-like receptor-8(TLR8) genetic polymorphisms and susceptibility to ischemic stroke (IS) in patients of different traditional Chinese medicine (TCM) pat-terns. Methods 311 IS patients of wind-phlegm stasis-obstruction pattern, 284 IS patients of qi-deficiency blood-stasis pattern and 605 controls were recruited. The real-time PCR technology was used to detect TLR8 gene expression levels. TLR8 rs3764880 single nucleotide polymorphism(SNPs) were genotyped using the Se-quenom Mass ARRAY platform. Levels of inflammatory cytokines were measured by using ELISA. Statistical analyses were carried out by using PLINK and SPSS 16. 0 software. Results TLR8 gene variant rs3764880 polymorphism was significantly associated with wind-phlegm stasis-obstruction pattern in IS males patients in the allele model (P<0. 05). In IS male patients of qi-deficiency blood-stasis pattern , level of IL-8 was high-er in the GG genotype carriers than the AA+AG genotype carriers (P<0. 05). The GG+AG genotype carri-ers had higher levels of TNF-α and IL-8 than the AA genotype carriers ( P <0. 05 ) . TLR8 gene variant rs3764880 polymorphism was significantly associated with the levels of TC (βb = -0. 03, Padj =0. 001) and LDL (βb= -0. 25, Padj =0. 018) in additive model in qi-deficiency blood-stasis pattern in Han Chinese IS male patients. Conclusion TLR8 gene rs3764880 polymorphism might be associated with the susceptibility to IS in patients of wind-phlegm stasis-obstruction and affect inflammatory reaction and lipid metabolism in Han Chinese males of qi-deficiency blood-stasis pattern.关键词
缺血性中风/中医证型/Toll样受体8/单核苷酸多态性/炎性细胞因子Key words
ischemic stroke/traditional Chinese medicine pattern/Toll-like receptor-8/single nucleotide pol-ymorphism/inflammatory cytokines分类
医药卫生引用本文复制引用
古联,周金英,谭金晶,梁宝云,严雁,陈清,谢娟娟,唐农..Toll样受体8基因rs3764880多态性与汉族男性缺血性中风的关联性研究[J].北京中医药大学学报,2016,(2):122-127,6.基金项目
国家自然科学基金资助项目(No.81260594,81473670),广西省自然科学基金项目(No.2013GXNSFAA019145),广西中医药大学第一附属医院青年科学基金课题(No. GZYQJ2014007),广西高等学校优秀中青年骨干教师培养工程资助成果 （No.81260594,81473670）
