北京中医药大学学报Issue(2)：151-154,4.DOI:10.3969/j.issn.1006-2157.2016.02.013
正交试验法优化珠子参总皂苷提取工艺
Optimization of extraction technology for total saponins from Rhizoma Panacis Majoris using orthogonal test
摘要
Abstract
Objective To optimize extraction technology for total saponins from Rhizoma Panacis Majoris ( zhuzishen) and provide a theoretical basis for development and utilization of saponins from Rhizoma Panacis Majoris. Methods Ultraviolet spectrophotometry was employed to detect content of total saponins from Rhizoma Panacis Majoris with detection wave length set at 540 nm. And effects of ethanol concentration, solvent quantity, extraction time and extraction times on extraction process were investiga-ted by using orthogonal test design. Results Linear range of total saponins from Rhizoma Panacis Majo-ris was 0. 052~0. 182 mg, and the average recovery rate was 98. 64%. The best extraction technology for total saponins from Rhizoma Panacis Majoris was to extract 3 times with 10 times treatment of 70%ethanol for 1 h per time. The average yield rate of total saponins from Rhizoma Panacis Majoris was 12. 32%. Conclusion This established ultraviolet spectrophotometry was accurate and reliable to detect contents of total saponins from Rhizoma Panacis Majoris. At the same time, this optimized extraction technology of total saponins from Rhizoma Panacis Majoris was simple, stable and feasible.关键词
珠子参/总皂苷/提取工艺/正交试验Key words
Rhizoma Panacis Majoris/total saponin/extract technology/orthogonal test分类
医药卫生引用本文复制引用
李凤英,王英姿,王晴,张秀婷,张春泥,李文华,骆声秀..正交试验法优化珠子参总皂苷提取工艺[J].北京中医药大学学报,2016,(2):151-154,4.基金项目
国家科技重大专项课题(No.2012ZX09103201-044) （No.2012ZX09103201-044）
