草业学报Issue(2)：77-86,10.DOI:10.11686/cyxb2015142
一株抗马铃薯坏疽病莫海威芽孢杆菌（Bacillus mojavensis ZA1）培养条件的优化
Optimization of culture conditions of a biocontrol agent Bacillus mojavensis ZA1 to antagonize Phoma foveata
摘要
Abstract
This work aimed to identify optimum fermentation shaker-flask culture conditions for Bacillus mo-javensis strain ZA1.Various single culture factors were combined according to a Box-Behnken design.The op-timum ratio of ingredients for the culture medium for ZA1was found to be:14.25 g ammonium chloride,19 g corn flour,and 237 g potato,in 1000 mL distilled water.The optimum cultivation conditions were pH 7.7, temperature 28℃,and shaken at 180 r/min for 36 h.Under these conditions,the viable count of B .mojavens-is strain ZA1 was 4.12×10 10 colony-forming units per mL (CFU/mL).Further testing using the central com-posite design method in a 10 L fermentation tank found the optimum for dissolved oxygen level and shaker rota-tion speed to be 60% saturation and 180 r/min respectively.Under these conditions,when fermentation time was 36 h in the fermentation tank,the viable count of B .mojavensis strain ZA1 reached 1.59×10 11 CFU/mL. The results of this study lay a solid foundation for the development of methodology for large scale production of biocontrol agents.关键词
莫海威芽孢杆菌/发酵工艺/优化Key words
Bacillus mojavensis/fermentation process/optimization引用本文复制引用
冯中红,畅涛,杨成德,薛莉,李旭,杨小利..一株抗马铃薯坏疽病莫海威芽孢杆菌（Bacillus mojavensis ZA1）培养条件的优化[J].草业学报,2016,(2):77-86,10.基金项目
国家自然科学基金（No．31160122）资助。 （）
