草业学报Issue(2)：95-104,10.DOI:10.11686/cyxb2015376
日粮硝酸盐水平对湖羊瘤胃硝态氮动态消失率、发酵参数及血液高铁血红蛋白含量的影响
Effects of dietary nitrate dosage on ruminal nitrate disappearance rate,fermentation parameters and concentration of blood methemoglobin of Hu Sheep
摘要
Abstract
This study investigated the effects of dietary nitrate dosage on ruminal nitrate disappearance rate, fermentation parameters and blood methemoglobin of Hu sheep.The experiment used a randomized block de-sign.Six selected Hu sheep were fed different levels of dietary potassium nitrate (0%,1%,2%,3%,4%, 5% of DM intake).The concentration of nitrate,nitrite and fermentation parameters in rumen fluid were ob-tained 0,0.5,1.0,1.5,2.0,4.0,6.0 and 8.0 h after feeding.In addition,the concentration of blood methe-moglobin was determined 2.0 h after feeding through blood collection from the jugular vein.The nitrate con-centration increased to a maximum value (0.95-2.14 g/L)at 1.0 h after feeding,then reduced rapidly.The nitrite concentration increased to maximum value (0.93 -6.22 μmol/L)1.5 h after feeding.The content of methemoglobin significantly increased with the nitrate addition (P <0.01),to a peak of 0.32% at 5% dietary potassium nitrate.Nitrate addition also significantly increased rumen fluid pH value,ammonia nitrogen con-centration and acetic acid/propionic acid 2.0 h after feeding (P <0.01).In conclusion,potassium nitrate addi-tion at over 3% of DM intake was found to reduce the concentration of microbial protein and total volatile fatty acids.Microbial protein synthesis and total volatile fatty acids were highest with 2% potassium nitrate addi-tion,and the dosage of 2% potassium nitrate was beneficial to rumen fermentation.关键词
微生物蛋白/硝酸盐还原/瘤胃发酵/添加量Key words
microbial protein/reducing nitrate/rumen fermentation/dosage引用本文复制引用
陈志远,马婷婷,方伟,左晓昕,林淼,赵国琦..日粮硝酸盐水平对湖羊瘤胃硝态氮动态消失率、发酵参数及血液高铁血红蛋白含量的影响[J].草业学报,2016,(2):95-104,10.基金项目
江苏省博士后科研资助计划（1402114C）和江苏高校优势学科建设工程资助项目资助。 （）
