草业学报Issue(2)：105-113,9.DOI:10.11686/cyxb2015466
用于羊草基因分型的 SNP 分子标记技术研究
A study of SNP molecular marker technology for Leymus chinensis genotyping
摘要
Abstract
Leymus chinensis ,is a widely distributed and often ecologically dominant perennial grass in China, frequently used as research material.Because we know of no previous reports of markers in L.chinensis ,we performed single nucleotide polymorphism (SNP)mining of 48 genotypes,based on the transcriptome data in the laboratory and established a method using high resolution melting (HRM)curves for L.chinensis SNP genotyping.A total of 41843 SNPs were detected from the transcriptome data by bioinformatics analysis. Transitions were nearly twice as frequent as transversions.Among 112 pairs of designed SNP primers,98 SNP primer pairs (87.5%)were able to amplify bright single bands,72 primer pairs (64.29%)had a suitable am-plified band size,and 46 SNP primer pairs (41.07%)were suitable for HRM genotyping.The 48 germplasm genotypes of L.chinensis could be separated by 7 primer pairs and the fingerprint of each identified.The partialHRM genotyping results were verified by sequencing.The results of genotype by sequencing analysis were con-sistent with the results of HRM genotyping,so confirming that the HRM genotyping was reliable.HRM for SNP genotyping first established in this study can also be used in other breeding work with L.chinensis ,inclu-ding parent selection,gene correlation analysis,molecular marker assisted breeding,development of functional molecular markers and new variety protection.关键词
羊草/转录组/SNP 标记/HRM 基因分型/指纹图谱Key words
Leymus chinensis/transcriptome/SNP marker/HRM genotyping/fingerprint引用本文复制引用
侯莉娟,齐晓,齐冬梅,陈双燕,刘公社..用于羊草基因分型的 SNP 分子标记技术研究[J].草业学报,2016,(2):105-113,9.基金项目
国家草品种区域试验项目－羊草新品种“三性”测试（农财发［2015］36号）和国家重点基础研究发展计划（973计划）项目（2014CB138704）资助。 （）
