草业学报Issue(2)：141-149,9.DOI:10.11686/cyxb2015122
秋水仙素处理清水苜蓿胚根对染色体倍性与叶表皮细胞的诱变效应
The mutagenic effects of colchicine treatment on chromosome ploidy and the leaf epi-dermal cells of radicles of Medicago sativa cv.Qingshui
摘要
Abstract
The objective of this experiment was to determine the appropriate colchicine dose levels to produce polyploids of Medicago sativa cv.Qingshui.Three different colchicine concentrations (0.05%,0.10%, 0.20%)and three treatment durations (4 h,8 h,12 h)were used to induce the radicle of gemmiferous seeds and changes were observed in the root tip cell chromosome number and in inclusions in the lower epidermis of leaves.The results showed that colchicine can change chromosomes in the root tip cells of alfalfa seedlings, producing a variety of different mutant types (4x <2n <8x ,2n =8x and 2n >8x ).The appropriate dose and treatment period of colchicine to induce a higher proportion of octaploid cells (2n =8x )is 0.1%+8h,with a mutation rate of 13.3%.Increasing levels of colchicine were associated with increases in the size (length, width,length/width)of guard cells and in the number of chloroplasts in the leaves’lower epidermis,while the density of stomata decreased.The size of guard cells and number of chloroplasts were largest in the 0.1%+8h, 0.1+12h and 0.2%+4h treatments,where stomatal densities were also relatively the smallest.The largest mutagenic effects were observed under the 0.1%+8h treatment.关键词
清水苜蓿/秋水仙素/染色体/叶片下表皮细胞Key words
Medicago sativa cv.Qingshui/colchicine/chromosome/lower epidermis cell of leafs引用本文复制引用
李悦,师尚礼..秋水仙素处理清水苜蓿胚根对染色体倍性与叶表皮细胞的诱变效应[J].草业学报,2016,(2):141-149,9.基金项目
农业部牧草种质资源保护项目（NB2130135）资助。 （）
