长春中医药大学学报Issue(1)：37-39,3.DOI:10.13463/j.cnki.cczyy.2016.01.011
丹参寒热药性的实验评价
Evaluating cold and hot characteristic of Salvia miltiorrhiza Bge. by experimental method
摘要
Abstract
Objective To evaluate the cold and hot characteristic ofSalvia miltiorrhiza Bge. by the method we have set up. Methods MTT assay was used to investigate the effect ofSalvia miltiorrhiza Bge. on the growth of SGC-7901 in vitro. Morphological changes of SGC-7901 treated withSalvia miltiorrhiza Bge. were observed through invert microscope. Trypan-blue staining was used to analyze the cytotoxicity ofSalvia miltiorrhiza Bge. Results Salvia milti-orrhiza Bge. can inhibit the growth and proliferation of SGC-7901 in the concentration range of 5-800 μg / mL. Morpho-logical observation showed Salvia miltiorrhiza Bge. in the concentration range we have chosen could decrease the densi-ty of SGC-7901 and condense the cell. Trypan-blue staining showedSalvia miltiorrhiza Bge. had no cytotoxicity on SGC-7901. Conclusion The results show thatSalvia miltiorrhiza Bge. is a cold drug and have no cytotoxicity.关键词
丹参/MTT 法/SGC-7901 细胞/台盼蓝染色Key words
Salvia miltiorrhiza Bge./MTT assay/SGC-7901/rypan-blue staining分类
医药卫生引用本文复制引用
王征,刘建利,王翠玲,唐志书,宋中兴..丹参寒热药性的实验评价[J].长春中医药大学学报,2016,(1):37-39,3.基金项目
国家自然科学基金（20872118）；教育部博士点基金（20070697012）；陕西省重大科技专项（2008ZDKG-67）；陕西省重点实验室基金（08JZ74，02JS15）。 （）
