广东牙病防治2016，Vol.24Issue(1)：20-25,6.DOI:10.12016/j.issn.2096-1456.2016.01.004
氧化锆陶瓷表面粗糙度对微生物附着的影响
The influence of the roughness of zirconia ceramic surface on microbial attachment
摘要
Abstract
Objective To analyze the influence of the zirconia ceramic surface roughness on microbial attachment. Methods The surface of zirconia ceramics was abraded progressively into three roughness levels of rough, medium rough and smooth surface by silicon carbide sand papers. Then the surface roughness, surface morphology and contact angle of zirconia ceramic were measured by atomic force microscopy and optical contact angle measuring device, which was fol-lowed by detecting the adhesive force of different roughness of zirconia ceramic by Streptococcus mutans UA159 prepro-cessed probe. At last scanning electron microscope and confocal laser scanning microscopy were used to observe biofilm formation after co-culture of zirconia ceramics and Streptococcus mutans. Results For different roughness of zirconia ce-ramic surface, with the smoother degree of their surfaces, the contact angle decreases step by step, surface energy increa-ses gradually, surface biofilm thickness decreases generally, and adhesive force between Streptococcus mutans and surface of zirconia ceramics decreases by degrees, whereas the biofilm bacteria mass of different roughness of zirconia ceramics does not have statistical difference. Conclusion Zirconia surface roughness affects the adhesion force of Streptococcus mutans which grows with the increase of surface roughness. The biofilm thickness and the mass of microorganisms less-ened correspondingly with the decreasing of surface roughness, until it drops to a certain number, from where the biofilm thickness and the mass of microorganisms remains stable.关键词
氧化锆/变异链球菌/粘附力/表面粗糙度/生物膜Key words
Zirconium oxide/Streptococcus mutans/Adhesive force/Surface roughness/Biofilm分类
医药卫生引用本文复制引用
余培,薛晶,张筱薇,郑苍尚..氧化锆陶瓷表面粗糙度对微生物附着的影响[J].广东牙病防治,2016,24(1):20-25,6.基金项目
深圳市科技研发基金项目(JCYJ201304-01111623884) （JCYJ201304-01111623884）
