广东牙病防治2016，Vol.24Issue(2)：79-87,9.DOI:10.12016/j.issn.2096-1456.2016.02.003
京尼平交联丝素蛋白—壳聚糖缓释微球的制备与表征
Preparation and characterization of genipin-crosslinked silk fibroin/chitosan sustained release microspheres
摘要
Abstract
Objective To investigate the efficiency of different concentrations of genipin and silk fibroin ( SF):chi-tosan ( CS) ratios on the package and release of SF-CS composite microspheres. Methods Selected the microspheres with a SF:CS ratio of 1:1 to encapsulate different concentrations of bovine serum albumin ( BSA) and compared their en-capsulation efficiency and sustained-release rate with those of pure chitosan microspheres. SF-CS microspheres were pre-pared with emulsion cross-linking technique. The microspheres were observed by scanning electron microscope. Size dis-tribution was measured by a laser particle size analyzer. X-ray diffractometry ( XRD) , fourier transform infrared spectros-copy ( FTIR) and thermogravimetricanalyzer ( TGA) were used to analyze their structural characteristics. BCA method was used for determination of the drug entrapment, loading rate and cumulative release of the total drug. Results The five types of microspheres ( m ( CS): m ( SF) = 1:0. 5, 0. 1 g or 0. 5 g genipin; m ( CS):m ( SF) = 1:1, 0. 05 g or 1 g ge-nipin; m ( CS):m ( SF) = 1:2, 0. 5 g eniping) had a more spherical shape and smooth surface with particle size between 70-147 μm. The microspheres prepared with m ( CS):m ( SF) of 1:1 and 0. 05 g genipin in the presence of 10 mg, 20 mg, or 50 mg BSA of BSA burst-released ( 30. 79 ± 3. 43)%, ( 34. 41 ± 4. 46)%, or ( 41. 75 ± 0. 96)% of the en-trapped BSA on the first day, and cumulatively released ( 75. 20 ± 2. 52)%, ( 79. 16 ± 4. 31)%, and ( 89. 04 ± 4. 68)%in 21 d, respectively. The pure CS microspheres prepared in the presence of 10 mg BSA burst-released ( 39. 53 ± 1. 76)% on the first day and cumulatively released ( 83. 57 ± 2. 33)% of the total encapsulated BSA in 21 d. Conclusion The SF-CS composite microspheres had a higher sustained release rate than the pure CS microspheres and may be a better drug carrier.关键词
丝素蛋白/壳聚糖/京尼平/微球/控释Key words
Silk fibroin/Chitosan/Genipin/Microspheres/Control release分类
医药卫生引用本文复制引用
叶漫文,方炜,石勇,盘杰,曾曙光..京尼平交联丝素蛋白—壳聚糖缓释微球的制备与表征[J].广东牙病防治,2016,24(2):79-87,9.基金项目
广东省自筹经费类科技计划项目粤科规财字[2015]110号 （）
