河北医学2016，Vol.22Issue(2)：180-184,5.DOI:10.3969/j.issn.1006-6233.2016.02.002
脂肪间质干细胞移植对肝硬化大鼠的实验研究
Cirrhotic Rat Model Treated with Adipose Mesenchymal Stem Cells
摘要
Abstract
Objective:To investigate the clinical effect of adipose mesenchymal stem cells transplanted from the portal vein and caudal vein graft clinical in treatment of liver cirrhosis in rats. Method:45 Wister rats data were analyzed, and using randomly controlled method, were divided into the control group, the por-tal transplantation group and tail vein graft group, with 15 in each group. The portal transplantation group and tailvein transplantation group were intraveously injected from the tail and portal vein 2mL intergovern-mental fat stem cell suspension with 2 × 106 cells, the control group injected 2mL cell culture medium, the treatment effect was observed on three groups of rats in six weeks. Result:Liver portal vein group blunt edge indicated thicker texture;The liver texture of the control group and the tail vein group was relatively stiff, liver atrophy and dark colors, there was different degrees of cirrhosis; of the three groups before treatment, AST, ALT, ALB and total bilirubin ( TBIL) index was not significantly different ( P>0.05);AST, ALT and TBLL index of the portal group were significantly lower than those of the control group and the tail vein group ( P <0.05);ALB significantly higher than that of control group and the tail vein group ( P <0.05);the liver of the control group under the microscope showed:there were different degrees of liver degeneration and nec-rosis, hepatic steatosis proportion accounted for more than 2/3 of lobules;liver fibrosis was found in tail vein group, and the number of cells was less than that of the control group;liver fibrosis of the portal group was lightest, normal liver cells could be seen under light microscope. Conclusion: Adipose mesenchymal stem cells are easily separated, cultured, and esily ferried and expressed with exogenous gene, belonging to the new seed cells in the treatment of cirrhosis with liver transplantation,after transplantation, it can effectively improve the body's liver function and prolong life of the body, which has important research value.关键词
脂肪间质干细胞/门静脉/尾静脉移植/大鼠肝硬化Key words
Adipose mesenchymal stem cells/Portal vein/Tail vein transplantation/Liver cir-rhosis引用本文复制引用
李蓉..脂肪间质干细胞移植对肝硬化大鼠的实验研究[J].河北医学,2016,22(2):180-184,5.基金项目
陕西省卫生厅科学研究基金项目,(编号:14H21) （编号:14H21）
