河北医学2016，Vol.22Issue(2)：261-264,4.DOI:10.3969/j.issn.1006-6233.2016.02.031
细胞DNA定量分析联合液基细胞学在宫颈癌筛查中的临床应用研究
Clinical Application of DNA Quantitative Analysis Combined with Liquid Based Cytology in Cervical Cancer Screening
摘要
Abstract
Objective:To study the clinical application of DNA quantitative analysis combined with liq-uid based cytology in cervical cancer screening. Method:From October 2013 to October 2014, 1496 cases of cervical cancer screening and biopsy results were selected in our hospital. In all cases, two pieces were prepared by using the cervical brush, and the liquid based method was used to carry out the PAP staining, which was used for routine TBS diagnosis. In addition, a Feulgen staining was used for quantitative analysis of DNA cells. Result:A total of 1496 cases of DNA were detected, Biopsy results showed 5 cases of carcino-ma, 40 cases of CIN1, 6 cases of CIN2, 5 cases of CIN3. Among them 6 cases of CIN2, and 6 cases of CIN2 were detected. The results showed that the cells were positive after quantitative analysis. The results of 5 ca-ses of CINl implementation after the DNA quantitative analysis showed suspicious, and then the biopsy was delayed. Using only conventional cytologic diagnosis, 4 cases of CIN2 were diagnosed as ascus, biopsy was delayed perhaps for being recommended for reviewing and then condition was delayed, especially two cases of cervical cancer were diagnosed with ASCUS. 12 cases of CINl were diagnosed as negative, 30 cases of CINl were disignosed as ASCUS. The sensitivity of DNA quantitative analysis method was significantly higher than that of conventional cytology in the detection of high grade cervical precancerous lesions. Once used in com-bination, the sensitivity was significantly improved. However, compared with the conventional cytology, the specificity of DNA quantitative analysis method was lower than that of conventional cytology. In the combined use of cases, specificity and conventional cytology were compared, although the specificity decreased, the sensitivity was further improved. Conclusion:DNA quantitative cytology and conventional liquid based cytol-ogy can effectively improve the detection rate of positive cells and the sensitivity of the detection of high-grade lesions. In combination of the two methods, the sensitivity was further improved, which can effectively improve the quality of cytology test and plays a more positive role in cervical cancer screening.关键词
细胞DNA定量/液基细胞学/宫颈癌/筛查Key words
DNA quantitative/Liquid based cytology/Cervical cancer/Screening/Applica-tion引用本文复制引用
谭景浪,陈琼,乔莎莎,覃勤英,邓娟荣,李美良,陆厚良..细胞DNA定量分析联合液基细胞学在宫颈癌筛查中的临床应用研究[J].河北医学,2016,22(2):261-264,4.基金项目
广西壮族自治区科学研究与技术开发计划项目,(编号:0993003B-29) （编号:0993003B-29）
