华西口腔医学杂志Issue(1)：17-22,6.DOI:10.7518/hxkq.2016.01.004
热休克蛋白27在烟草烟雾提取物介导的牙龈成纤维细胞损伤中的表达变化
Expression of heat shock protein 27 in cigarette smoke extract-induced injury of human gingival fibroblasts
摘要
Abstract
Objective This research aimed to observe the expression of heat shock protein 27 (HSP27) in human gingival fibroblasts (HGFs) cell injury induced by different concentrations of cigarette smoke extract (CSE). Methods The third to eighth generations of cultured HGFs were treated with serially diluted CSE of different concentrations (0, 2.5%, 5.0%, 12.5%, 25.0%, 50.0%). Wound-healing assay was performed to determine the migration of HGFs, and Western blot was used to determine the expression of HSP27. Results The migration capability of HGFs weakened with the increase of CSE concentration. HSP27 expression was negative in normal HGFs but positive in CSE-intervened HGFs in a concentration-dependent manner. Conclusion HSP27 concentration increased in the CSE-induced injury of HGFs. This finding suggests that HSP27 plays an important role in CSE-induced epithelial injury.关键词
烟草烟雾提取物/热休克蛋白27/人牙龈成纤维细胞/上皮损伤Key words
cigarette smoke extract/heat shock protein 27/human gingival fibroblasts/epithelial injury分类
生物科学引用本文复制引用
周芹,孙睿男,朱震坤..热休克蛋白27在烟草烟雾提取物介导的牙龈成纤维细胞损伤中的表达变化[J].华西口腔医学杂志,2016,(1):17-22,6.基金项目
中国博士后科学基金（2012M511524）；山东省博士后创新项目专项资金（2012030407）；中国博士后科学基金特别资助项目（2013T60679）；山东省医药卫生科技发展计划基金 （）
