临床肝胆病杂志Issue(1)：139-142,4.DOI:10.3969/j.issn.1001-5256.2016.01.027
基于GC-TOFMS和UPLC-QTOFMS代谢组学分析平台对轻微型肝性脑病特异性代谢物谱的分析
Analysis of specific metabolite profiling of minimal hepatic encephalopathy based on metabolomics analytical plat-forms of GC-TOFMS and UPLC-QTOFMS
摘要
Abstract
Objective To analyze and compare the serum metabolite profiling of healthy volunteers and patients with minimal hepatic en-cephalopathy(MHE),identifythespecificbiomarkersofMHE,andexplorethemethodforearlydiagnosisofMHE.Methods Twenty-nine patients with MHE caused by hepatitis B cirrhosis who were admitted to First Affiliated Hospital of Guangxi University of Chinese Medi-cine (MHE group)from October 2013 to October 2014,as well as 50 healthy volunteers who received physical examinations in this hospital (NC group)during the same period,were collected.Gas chromatography and time-of-flight mass spectrometry (GC-TOFMS)and ultra-high-pressure liquid chromatography-quadrupole-time-of-flight mass spectrometry (UPLC-QTOFMS)were applied to determine the metabolites in serum samples.Principal component analysis (PCA)and orthogonal partial least squares-discriminant analysis (OPLS-DA)were applied for multivariate statistical analysis,and univariate t test was applied for comparison between the two groups,so as to deter-minethebiomarkersforMHE.Results IncombinationofthemodelinganalysiswithPCAandOPLS-DA,theresultsfromunivariatet test,and vasoactive intestinal peptide (VIP)value in the models,a total of 27 possible specific biomarkers for MHE were found in serum in the two groups.According to the fold change between MHE group and NC group in OPLS-DA model,there were 9 down-regulated metab-olitesand18up-regulatedmetabolitesinthepatientswithMHEcomparedwithhealthyvolunteers.Conclusion Serummetabolomicsana-lytical platforms such as GC-TOFMS and UPLC-QTOFMS can detect a variety of metabolites in serum,which can be of help to explore the pathogenesis of MHE.In addition,the diagnostic model OPLS-DA established in this study can differentiate between the normal population and patients with MHE,which may be applied for early diagnosis of MHE.关键词
肝性脑病/质谱分析法/早期诊断Key words
hepatic encephalopathy/mass spectrometry/early diagnosis分类
医药卫生引用本文复制引用
黄国初,王萌,杨小徽,姚凡,钟宇,姚春..基于GC-TOFMS和UPLC-QTOFMS代谢组学分析平台对轻微型肝性脑病特异性代谢物谱的分析[J].临床肝胆病杂志,2016,(1):139-142,4.基金项目
国家自然科学基金项目 （）
