临床神经外科杂志2016，Vol.13Issue(1)：40-44,5.DOI:10.3969/j.issn.1672-7770.2016.01.011
β-catenin 介导蛋白激酶 D2 对脑胶质瘤细胞增殖的影响
Involvement of β-catenin in effect of protein kinase D2 on glioma cell proliferation
摘要
Abstract
Objective To study whether protein kinase ( PKD2 ) affect glioma proliferation by regulating β-catenin.Methods The effect of PKD2 or β-catenin down-regulation on the proliferation of glioma cells was detected by EDU incorporation assay .Western blotting was applied to detect totalβ-catenin expression level and β-catenin level in each subcellular fraction ,such as the membrane, nuclear and cytosolic fractions of the cells after down-regulation of PKD2. Results Compared with the control group , the proliferation of glioma cells decreased in PKD 2 down-regulation group ( P<0 .01 ) .The total β-catenin protein level did not change , but the cytosolic fractions increased , while those in the nuclear fraction decreased after PKD 2 down-regulation ( P<0.01).Conclusion Down-regulation of PKD2 may inhibit glioma cell proliferation by decreasing β-catenin level in the nuclear fraction .关键词
胶质瘤/蛋白激酶D2/细胞增殖/β-连环蛋白Key words
glioma/protein kinase D2/cell proliferation/β-catenin分类
医药卫生引用本文复制引用
宋旭,王猛,华磊,陆冬,石琼,周秀萍,于如同..β-catenin 介导蛋白激酶 D2 对脑胶质瘤细胞增殖的影响[J].临床神经外科杂志,2016,13(1):40-44,5.基金项目
国家自然科学基金(81272777 （）
81372699 （）
81472345) （）
