泸州医学院学报Issue(1)：21-25,5.DOI:10.3969/j.issn.1000-2669.2016.01.002
二肽基肽酶-IV介导内皮间质转化在糖尿病肾纤维化过程中的作用及机制研究
Effect and mechanisms of dipeptidyl peptidase-IV on endothelial-to-mesenchymal transition in diabetic kidney fibrosis
摘要
Abstract
Objective To analyze the roles and mechanisms of dipeptidyl peptidase-IV (DPP-IV) on endothelial-to-mesenchymal transition in diabetic kidney fibrosis. Methods Diabetic CD1 mice were used as chronic diabetic kidney fibrosis model. Mice were treated with or without DPP-IV inhibitor for 4 weeks, kidney samples from each groups were used to analyze the pathological changes, DPP-IV protein levels, and endothelial-to-mesenchymal transition. In vitro, human microvascular endothelial cells were stimulated with or without TGFβ2, and after treatment with DPP-IV inhibitor or siRNA, cell migration, DPP-IV protein levels, endothelial-to-mesenchymal transition, and TGFβ/smad signaling were analyzed. Results: Diabetic CD-1 mice exhibited significant kidney fibrosis and high levels of DPP-IV expression when compared with control mice. DPP-IV inhibitor-treated diabetic mice exhibited a suppression of DPP-IV protein expression and an amelioration of kidney fibrosis associated with the inhibition of EndoMT. In cultured endothelial cells, we found that DPP-IV inhibitor inhibited TGFβ2-induced EndoMT and TGFβ/Smad signaling. Conclusion: DPP-IV inhibitor can ameliorate kidney fibrosis by suppressing EndoMT and TGFβ/Smad signaling.关键词
二肽基肽酶-IV/糖尿病肾纤维化/内皮间质转化Key words
Dipeptidyl peptidase-IV/Diabetic kidney fibrosis/Endothelial-to-mesenchymal transition分类
医药卫生引用本文复制引用
宋丽,孙玉红,施森..二肽基肽酶-IV介导内皮间质转化在糖尿病肾纤维化过程中的作用及机制研究[J].泸州医学院学报,2016,(1):21-25,5.基金项目
国家自然科学基金项目 （）
