泸州医学院学报Issue(1)：31-33,3.DOI:10.3969/j.issn.1000-2669.2016.01.004
一种有效急性酶分离大鼠心肌细胞及复钙的方法
An effective method of acute isolation of cardiomyocytes and calcium recovery in rats
摘要
Abstract
Objective To establish a simple and efficient method for acute isolation of myocardial cells and effective recovery of calcium in rats, so as to provide a favorable prerequisite protection of cardiomyocytes for further downstream studies of the physiological mechanism of single myocardial cell and many other experiments. MethodsUsing type II collagenase in Langendorff irrigation flow device under constant pressure or constant flow perfusion, cardiomyocytes were isolated from rats, followed by a three-step calcium recovery was applied. Results Cardiomyocytes were successfully isolated from rats using Langendorff irrigation flow system at a constant pressure or constant flow perfusion of collagenase type II, which showed a clear cross striations and strong three-dimensional sense of myocardial cells. After calcium recovery, the survival rate of cardiomyocytes remains as high as 50% in Tyrode solution. Conclusion A new and high efficient method of acute isolation of cardiomyocytes in rats was established successfully, which can provide functional single cardiomyocyte for further downstream studies.关键词
大鼠/心肌细胞/LangendorffKey words
Rat/Cardionyocyte/Langendorff分类
医药卫生引用本文复制引用
程俊,曾晓荣,谭晓秋,李鹏云,文静,毛亮,杨艳..一种有效急性酶分离大鼠心肌细胞及复钙的方法[J].泸州医学院学报,2016,(1):31-33,3.基金项目
国家自然科学基金资助项目(No：81173661)，泸州市-泸州医学院联合专项 （No：81173661）
